Department of Biochemistry, Faculty of Medicine, Adnan Menderes University, Adnan Menderes Universitesi Bilim ve Teknoloji Araştirma ve Uygulama Merkezi (ADU-BILTEM), 09100, Aydin, Turkey.
Mod Rheumatol. 2010 Feb;20(1):34-9. doi: 10.1007/s10165-009-0230-9. Epub 2009 Sep 29.
Ankylosing spondylitis (AS) is a chronic inflammatory disease mainly affecting the spine and sacroiliac joints. Mediators such as macrophage migration inhibitory factor (MIF) and interleukin-10 (IL-10) are thought to be involved in several inflammatory conditions, including AS. Proinflammatory cytokines regulate the production of oxidative stress markers, such as nitric oxide (NO) and malondialdehyde (MDA). Although oxidative stress and lipid peroxidation have been reported in AS, the association of AS with commonly known oxidative stress markers and cytokines remains uncertain. We have therefore studied whether serum MIF levels are elevated in patients with AS and whether the levels correlate with oxidative stress markers and disease activity parameters. Twenty-five AS patients and 18 healthy controls participated in this study; subjects with hypertension, diabetes, hyperlipidemia, and obesity were excluded. The levels of acute phase reactants, serum levels of glucose, lipids, MIF, IL-10, NO and MDA were studied. Spinal mobility was assessed by the Bath Ankylosing Spondylitis Metrology Index (BASMI). Patients were also assessed using with the Bath Ankylosing Spondylitis Functional Index and the Bath Ankylosing Spondylitis Disease Activity Index. Age and sex distribution were found to be comparable between AS patients and controls (p > 0.05). Acute phase reactants and MIF levels were significantly higher (p < 0.05) and IL-10 levels were significantly lower (<0.001) in the AS patients than in controls. There was a significant correlation between BASMI and MIF levels in AS patients (r = 0.714, p < 0.001). Based on these results, MIF may be involved in the pathogenesis of the chronic inflammation in AS and, consequently, targeting MIF may be beneficial in preventing complications or in initiating early treatment of the disease.
强直性脊柱炎（AS）是一种主要影响脊柱和骶髂关节的慢性炎症性疾病。巨噬细胞移动抑制因子（MIF）和白细胞介素-10（IL-10）等介质被认为参与了多种炎症性疾病，包括 AS。促炎细胞因子调节氧化应激标志物的产生，如一氧化氮（NO）和丙二醛（MDA）。尽管 AS 中已经报道了氧化应激和脂质过氧化，但 AS 与常见的氧化应激标志物和细胞因子的关联仍不确定。因此，我们研究了 AS 患者血清 MIF 水平是否升高，以及这些水平是否与氧化应激标志物和疾病活动参数相关。本研究纳入了 25 例 AS 患者和 18 例健康对照者；排除了高血压、糖尿病、高血脂和肥胖患者。研究了急性期反应物、血清葡萄糖、脂质、MIF、IL-10、NO 和 MDA 水平。通过 Bath 强直性脊柱炎计量学指数（BASMI）评估脊柱活动度。还使用 Bath 强直性脊柱炎功能指数和 Bath 强直性脊柱炎疾病活动指数对患者进行评估。AS 患者和对照组的年龄和性别分布无差异（p > 0.05）。AS 患者的急性期反应物和 MIF 水平显著升高（p < 0.05），IL-10 水平显著降低（<0.001）。AS 患者的 BASMI 与 MIF 水平呈显著正相关（r = 0.714，p < 0.001）。基于这些结果，MIF 可能参与了 AS 慢性炎症的发病机制，因此靶向 MIF 可能有助于预防并发症或早期治疗疾病。
