Division of Biophysics and Bioimaging, University Health Network, 610 University Ave., Toronto, ON M5G 2M9, Canada.
J Biophotonics. 2009 Nov;2(11):669-81. doi: 10.1002/jbio.200910079.
The use of injected stem cells, leading to regeneration of ischemic heart tissue, for example, following coronary artery occlusion, has emerged as a major new option for managing 'heart attack' patients. While some clinical trials have been encouraging, there have also been failures and there is little understanding of the multiplicity of factors that lead to the outcome. In this overview paper, the opportunities and challenges in applying biophotonic techniques to regenerative medicine, exemplified by the challenge of stem cell therapy of ischemic heart disease, are considered. The focus is on optical imaging to track stem cell distribution and fate, and optical spectroscopies and/or imaging to monitor the structural remodeling of the tissue and the resulting functional changes. The scientific, technological, and logistics issues involved in moving some of these techniques from pre-clinical research mode ultimately into the clinic are also highlighted.
例如，在冠状动脉阻塞后，利用注射的干细胞促进缺血性心脏组织再生，已成为治疗“心脏病发作”患者的一种主要新选择。虽然一些临床试验令人鼓舞，但也有失败的情况，而且对于导致这种结果的多种因素知之甚少。在这篇综述论文中，我们考虑了将生物光子技术应用于再生医学所面临的机遇和挑战，以缺血性心脏病的干细胞治疗为例。重点是光学成像以跟踪干细胞的分布和命运，以及光学光谱学和/或成像是为了监测组织的结构重塑和由此产生的功能变化。我们还强调了将这些技术中的一些从临床前研究模式最终推向临床应用所涉及的科学、技术和后勤问题。
