Antifertility and Infertility Research Center, Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences & Research (DISPAR), New Delhi, India.
Immunopharmacol Immunotoxicol. 2010 Mar;32(1):92-8. doi: 10.1080/08923970903164300.
In this study, the negative effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD) on the immune system and body weight gain of rats and the preventive effects of curcumin were examined. For this purpose, 3-4 months old 128 Wistar albino rats with 280-310g body weights were used. The 2microg/kg dose of 2,3,7,8-TCDD and 100mg/kg dose of curcumin were dissolved in corn oil and orally given to the rats found in the experimental and control groups. Then, the serum samples were taken from all rats at 15, 30, 45 and 60th days to analyzed for the determination of TNF-alpha, IFN-gamma, IL-12 and IL-13 levels by ELISA method. The data of body weight gain was measured at 15, 30, 45 and 60th days. The results indicated that 2,3,7,8,3,7,8-TCDD caused to increase significantly (p<0.05) in serum TNF-alpha levels. However, it caused significantly (p<0.05) decreases in the levels of IFN-gamma, IL-12 and IL-13 in rats. On contrary, curcumin increased IFN-gamma, IL-12 and IL-13 levels, but decreased TNF-alpha level in rats. Additionally, TCDD caused significantly (P<0.01) reductions in the body weight gain. However curcumin reversed this effect of TCDD.In conclusion, 2,3,7,8-TCDD significantly suppressed the humoral immunity and body weight gain in rats at doses of 2microg/kg. However curcumin, which was found in some plants, eliminated the effect of TCDD on immune system and body weight when it was given together with 2,3,7,8-TCDD. It is thought that this effect may have occurred via curcumin and TCDD were binding aryl hydrocarbon receptor (AhR) competitively.
在这项研究中，研究了 2,3,7,8-四氯二苯并对二恶英（TCDD）对大鼠免疫系统和体重增加的负面影响，以及姜黄素的预防作用。为此，使用了 3-4 个月大、体重 280-310g 的 128 只 Wistar 白化大鼠。2μg/kg 剂量的 2,3,7,8-TCDD 和 100mg/kg 剂量的姜黄素溶解在玉米油中，分别给予实验组和对照组的大鼠口服。然后，在第 15、30、45 和 60 天从所有大鼠中抽取血清样本，通过 ELISA 法分析 TNF-α、IFN-γ、IL-12 和 IL-13 水平。体重增加数据在第 15、30、45 和 60 天测量。结果表明，2,3,7,8-TCDD 导致血清 TNF-α 水平显著升高（p<0.05）。然而，它导致大鼠 IFN-γ、IL-12 和 IL-13 水平显著降低（p<0.05）。相反，姜黄素增加了大鼠 IFN-γ、IL-12 和 IL-13 水平，但降低了 TNF-α 水平。此外，TCDD 导致体重增加显著减少（P<0.01）。然而，姜黄素逆转了 TCDD 的这种作用。总之，2,3,7,8-TCDD 以 2μg/kg 的剂量显著抑制了大鼠的体液免疫和体重增加。然而，姜黄素是在一些植物中发现的，当与 2,3,7,8-TCDD 一起给予时，消除了 TCDD 对免疫系统和体重的影响。据认为，这种作用可能是通过姜黄素和 TCDD 竞争性结合芳香烃受体（AhR）而发生的。
