Department of Pharmaceutical Toxicology, Faculty of Pharmacy, University of Inonu, Malatya, Turkey.
Immunopharmacol Immunotoxicol. 2010 Mar;32(1):99-104. doi: 10.3109/08923970903164318.
In this study, the negative effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the immune system and body weight gain of rats and the preventive effects of curcumin were examined.
For this purpose, 128 3-4-month-old Wistar albino rats with 280-310 g body weights were used. The 2 microg/kg dose of 2,3,7,8-TCDD and 100 mg/kg dose of curcumin were dissolved in corn oil and orally given to the rats found in the experimental and control groups. Then, the serum samples were taken from all rats at 15, 30, 45 and 60 days to analyzed for the determination of TNF-alpha, IFN-gamma, IL-12 and IL-13 levels by ELISA method. The data of body weight gain was measured at 15, 30, 45 and 60 days.
The results indicated that 2,3,7,8-TCDD caused a significant increase (p<0.05) in serum TNF-alpha level. However, it caused significant (p<0.05) decreases in the levels of IFN-gamma, IL-12 and IL-13 in rats. On the contrary, curcumin increased IFN-gamma, IL-12 and IL-13 levels, but decreased TNF-alpha level in rats. Additionally, TCDD caused significant (P<0.01) reductions in the body weight gain. However, curcumin reversed this effect of TCDD.
2,3,7,8-TCDD significantly suppressed the humoral immunity and body weight gain in rats at doses of 2 microg/kg. However curcumin, which was found in some plants, eliminated the effect of TCDD on immune system and body weight when it was given together with 2,3,7,8-TCDD. It is thought that this effect may have occurred via curcumin and TCDD binding aryl hydrocarbon receptor (AhR) competitively.
本研究旨在探讨 2,3,7,8-四氯二苯并对二恶英（TCDD）对大鼠免疫系统和体重增加的负面影响，以及姜黄素的预防作用。
为此，我们使用了 128 只 3-4 个月龄、体重 280-310g 的 Wistar 白化大鼠。将 2μg/kg 剂量的 2,3,7,8-TCDD 和 100mg/kg 剂量的姜黄素溶解在玉米油中，分别给实验组和对照组大鼠口服。然后，在第 15、30、45 和 60 天从所有大鼠中抽取血清样本，通过 ELISA 法分析 TNF-α、IFN-γ、IL-12 和 IL-13 水平。在第 15、30、45 和 60 天测量体重增加数据。
结果表明，TCDD 导致血清 TNF-α水平显著升高（p<0.05）。然而，它导致大鼠 IFN-γ、IL-12 和 IL-13 水平显著降低（p<0.05）。相反，姜黄素增加了大鼠 IFN-γ、IL-12 和 IL-13 水平，但降低了 TNF-α水平。此外，TCDD 导致大鼠体重显著减轻（P<0.01）。然而，姜黄素逆转了 TCDD 的这种作用。
TCDD 在 2μg/kg 剂量下显著抑制大鼠的体液免疫和体重增加。然而，在与 2,3,7,8-TCDD 一起给予时，在一些植物中发现的姜黄素消除了 TCDD 对免疫系统和体重的影响。据认为，这种作用可能是通过姜黄素和 TCDD 与芳香烃受体（AhR）竞争性结合而发生的。
