Choresca C H, Koo O J, Hong S G, Oh H J, Gomez D K, Kim J H, Lee B C, Park S C
Laboratory of Aquatic Animal Medicine, Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul, South Korea.
Reprod Domest Anim. 2010 Oct;45(5):e73-7. doi: 10.1111/j.1439-0531.2009.01525.x.
Several studies have previously been conducted regarding cell cycle synchronization in mammalian somatic cells. However, limited work has been performed on the control of cell cycle stages in the somatic cells of fish. The aim of this study was to determine the cell cycle arresting effects of several dimethyl sulfoxide (DMSO) concentrations for different times on different cell cycle stages of goldfish caudal fin-derived fibroblasts. Results demonstrated that the cycling cells or control group (68.29%) yields significantly higher (p < 0.05) arrest in G0/G1 phase compared with the group treated for 24 h with different concentrations (0.5%, 1.0% or 1.5%) of DMSO (64.88%, 65.70%, 64.22% respectively). The cell cycle synchronization in the treatment of cells with 1.0% DMSO at 48 h (81.14%) was significantly higher than that in the groups treated for 24 h (76.82%) and the control group (77.90%). Observations showed that treatment of DMSO resulted in an increase in the proportion of cells at G0/G1 phase for 48 h of culture. However, high levels of apoptotic cells can be detected after 48 h of culture treated with 1% concentration of DMSO.
此前已经进行了多项关于哺乳动物体细胞中细胞周期同步化的研究。然而，在鱼类体细胞的细胞周期阶段控制方面所做的工作有限。本研究的目的是确定不同浓度的二甲基亚砜（DMSO）在不同时间对金鱼尾鳍来源的成纤维细胞不同细胞周期阶段的细胞周期阻滞作用。结果表明，与用不同浓度（0.5%、1.0%或1.5%）的DMSO处理24小时的组（分别为64.88%、65.70%、64.22%）相比，循环细胞或对照组（68.29%）在G0/G1期的阻滞率显著更高（p < 0.05）。在48小时用1.0%DMSO处理的细胞的细胞周期同步化率（81.14%）显著高于处理24小时的组（76.82%）和对照组（77.90%）。观察结果显示，DMSO处理导致培养48小时后处于G0/G1期的细胞比例增加。然而，在用1%浓度的DMSO处理培养后48小时可检测到高水平的凋亡细胞。
