Choresca Casiano H, Koo Ok Jae, Oh Hyun Ju, Hong So Gun, Gomez Dennis K, Kim Ji Hyung, Lee Byeong Chun, Park Se Chang
Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea.
Cell Biol Int. 2009 Jan;33(1):65-70. doi: 10.1016/j.cellbi.2008.09.015. Epub 2008 Oct 17.
One of the most important factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We investigated the effects of serum starvation, culturing to confluence and roscovitine treatment on the cell cycle synchronization of goldfish caudal fin-derived fibroblasts by flow cytometric analysis. The results show that culturing the cells to confluence (85.5%) and roscovitine treatment (82.71%) yield a significantly higher percentage of cells arrested in the G0/G1 (P<0.05) phase than serum starvation (62.85%). Different concentrations of roscovitine (5, 10, or 15 microM) induce cell cycle arrest at the G0/G1 phase.
决定克隆胚胎发育成功的最重要因素之一是供体细胞的细胞周期阶段。我们通过流式细胞术分析研究了血清饥饿、培养至汇合状态以及用罗哌卡因处理对金鱼尾鳍来源的成纤维细胞细胞周期同步化的影响。结果表明，将细胞培养至汇合状态（85.5%）和用罗哌卡因处理（82.71%）导致停滞在G0/G1期的细胞百分比显著高于血清饥饿处理（62.85%）（P<0.05）。不同浓度的罗哌卡因（5、10或15微摩尔）诱导细胞周期停滞在G0/G1期。
