早期胚胎形态评估的观察者内和观察者间分析。
Intra- and inter-observer analysis in the morphological assessment of early-stage embryos.
机构信息
Leuven University Fertility Centre, UZ Leuven, Campus Gasthuisberg, Herestraat 49, 3000 Leuven, Belgium.
出版信息
Reprod Biol Endocrinol. 2009 Sep 29;7:105. doi: 10.1186/1477-7827-7-105.
BACKGROUND
The aim of this study was to determine the intra- and inter-observer variability in the evaluation of embryo quality. Multilevel images of embryos on day 1, day 2 and day 3, were analysed using different morphological parameters.
METHODS
Multilevel images of embryos on day 1, day 2 and day 3, were analysed using a standard scoring system. The kappa coefficient was calculated to measure intra- and inter-observer variability before and after training sessions.
RESULTS
Good to excellent intra-observer agreement was present for most parameters exceptions being scoring the position of pronuclei and the presence of a cytoplasmic halo on day 1, multinucleation on day 2 and the size of fragments on day 3. Inter-observer agreement was only good to excellent for the number of blastomeres on day 2 and day 3 and the orientation of the cleavage axes on day 2. Training sessions had a positive impact on inter-observer agreement.
CONCLUSION
In conclusion, assessment of morphological characteristics of early stage embryos using multilevel images was marked by a high intra-observer and a moderate inter-observer agreement. Training sessions were useful to increase inter-observer agreement.
背景
本研究旨在确定胚胎质量评估的观察者内和观察者间的可变性。使用不同的形态学参数对第 1、2 和 3 天的胚胎的多层次图像进行了分析。
方法
使用标准评分系统对第 1、2 和 3 天的胚胎的多层次图像进行了分析。计算了kappa 系数，以衡量培训前后的观察者内和观察者间的可变性。
结果
除了在第 1 天评估原核的位置和细胞质晕的存在、第 2 天多核和第 3 天碎片的大小等参数外，大多数参数的观察者内一致性良好到优秀。第 2 和第 3 天的卵裂球数量和第 2 天的卵裂轴方向的观察者间一致性仅为良好到优秀。培训课程对观察者间的一致性有积极的影响。
结论
总之，使用多层次图像评估早期胚胎的形态特征具有较高的观察者内一致性和中等的观察者间一致性。培训课程有助于提高观察者间的一致性。
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