Tatsuzuki Ayano, Ezaki Taichi, Makino Yasuo, Matsuda Yoshio, Ohta Hiroaki
Department of Obstetrics and Gynecology, Tokyo Women's Medical University, Tokyo, Japan.
Arch Histol Cytol. 2009 Mar;72(1):35-49. doi: 10.1679/aohc.72.35.
The general sugar expression pattern was studied in 9 normal full-term human placentas by the use of 21 individual lectins in combination with immunohistochemistry for various markers to understand the function of the placenta as the site of feto-maternal interactions. In mature intermediate and terminal villi, the brush border of the syncytiotrophoblast layer strongly expressed GlcNAc (as stained by WGA, S-WGA, DSL lectins) but weakly expressed sialic acid (Mal II, SNA). The cytoplasm of the syncytiotrophoblast layer showed weak expressions of GlcNAc and Gal/GalNAc with granular patterns. The cytotrophoblast layer, as also recognized by PCNA and HAI-1, typically expressed GlcNAc (LEL etc.) and Gal/GalNAc (MAL I). We found that the cytotrophoblast layer became very thin but largely maintained its continuity in the mature villi. The basement membranes of both the trophoblast layer and the endothelial layer strongly and continuously expressed mannose (Con A, LCA) and galactose (ECL, RCA I). Although endothelial cells almost exclusively expressed sialic acid and fucose, UEA I showed a heterogeneous reactivity with endothelial cells within the same vessels. No uniform expression pattern of any sugar was seen in stromal components except for Hofbauer cells, which usually expressed GlcNAc (LEL and DSL etc.). Thus, the sugar expression analysis by lectin histochemistry combined with immunohistochemistry proved helpful to understand the sugar chain related functions of the placenta under both normal and pathological conditions.
通过使用21种单独的凝集素结合针对各种标志物的免疫组织化学方法，在9个正常足月人胎盘中研究了一般的糖表达模式，以了解胎盘作为母胎相互作用部位的功能。在成熟的中间绒毛和终末绒毛中，合体滋养层的刷状缘强烈表达GlcNAc（如WGA、S-WGA、DSL凝集素染色），但唾液酸表达较弱（Mal II、SNA）。合体滋养层的细胞质中GlcNAc和Gal/GalNAc呈弱表达，呈颗粒状。细胞滋养层，如PCNA和HAI-1所识别的，典型地表达GlcNAc（LEL等）和Gal/GalNAc（MAL I）。我们发现，在成熟绒毛中细胞滋养层变得非常薄，但在很大程度上保持了其连续性。滋养层和内皮细胞层的基底膜强烈且持续地表达甘露糖（Con A、LCA）和半乳糖（ECL、RCA I）。尽管内皮细胞几乎只表达唾液酸和岩藻糖，但UEA I对同一血管内的内皮细胞显示出异质性反应。除了霍夫鲍尔细胞通常表达GlcNAc（LEL和DSL等）外，在基质成分中未观察到任何糖的统一表达模式。因此，凝集素组织化学结合免疫组织化学进行的糖表达分析被证明有助于了解正常和病理条件下胎盘的糖链相关功能。
