鉴定和表征霍乱弧菌非 O1、O139 菌株的噬菌体 PS166 溶原菌。

Identification and characterization of phage PS166 lysogens from non-O1, O139 strains of Vibrio cholerae.

机构信息

Department of Infectious Diseases, Indian Institute of Chemical Biology, Kolkata, India.

出版信息

Med Sci Monit. 2009 Oct;15(10):BR281-288.

PMID:19789504

Abstract

BACKGROUND

In recent years, non-O1, O139 serogroups of Vibrio cholerae have become a major source of pathogenic infection. However, the origin and acquisition of their virulence properties remain under explored. In this regard bacteriophages of Vibrio cholerae are well known to be the carriers of pathogenic traits across various strains. So, any possible association of vibriophages and non-O1, O139 serogroups would provide a deeper insight of their pathogenic threats.

MATERIAL/METHODS: Ten non-O1, O139 clinical isolates of Vibrio cholerae were induced by mitomycin C. Virulence profiles of those isolates were determined by multiplex PCR. BglII, KpnI and HaeII were used to generate the restriction profile of isolated bacteriophage. Two of the phage harboring strains was ribotyped by Southern hybridization.

RESULTS

In the present study, ten non-O1, O139 diarrheal isolates of Vibrio cholerae were examined for their ability to produce infectious phage particles out of which two strains, PG128 and PG130 were found to be positive. The host range and restriction profile of phage particles were identical to a biotype converting temperate vibriophage PS166. Both PG128 and PG130 carried unique ribotype pattern and lacked the major virulence determinants. But PG128 was found to carry hlyA, mshA, rtxC and toxR, a set of accessory virulence determinants.

CONCLUSIONS

The evidences present here provide definite clues for a possible phage mediated emergence of newer Vibrio choleare pathogens.

摘要

背景

近年来，非 O1、O139 血清群霍乱弧菌已成为主要的致病感染源。然而，它们毒力特性的起源和获得仍未得到充分探索。在这方面，霍乱弧菌噬菌体是众所周知的，可在各种菌株中携带致病特征。因此，噬菌体与非 O1、O139 血清群之间的任何可能关联，都将为其致病威胁提供更深入的了解。

材料/方法：用丝裂霉素 C 诱导 10 株非 O1、O139 临床分离霍乱弧菌。通过多重 PCR 确定这些分离株的毒力谱。使用 BglII、KpnI 和 HaeII 生成分离噬菌体的限制图谱。用 Southern 杂交对携带噬菌体的两种菌株进行核糖体分型。

结果

在本研究中，对 10 株非 O1、O139 腹泻性霍乱弧菌分离株进行了检测，以确定它们产生感染性噬菌体颗粒的能力，其中 2 株 PG128 和 PG130 呈阳性。噬菌体的宿主范围和限制图谱与生物型转化温和噬菌体 PS166 相同。PG128 和 PG130 均携带独特的核糖体型，缺乏主要毒力决定因素。但 PG128 携带 hlyA、mshA、rtxC 和 toxR，这是一组辅助毒力决定因素。