Woodward M J, Wray C
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, Great Britain.
Vet Microbiol. 1990 Oct;25(1):55-65. doi: 10.1016/0378-1135(90)90093-b.
DNA gene probes specific for genes encoding heat labile enterotoxin (LTI), heat stable enterotoxins (STIa, STII), vero cytotoxins (VT1, VT2), and adhesins K88 (F4), K99(F5), F41 and 987P(F6) were used to examine 873 isolates of E. coli from cases of diarrhoea (680 from pigs, 187 from cattle and six from sheep). A total of 188 were toxin gene positive and of these 84 belonged to the classical ETEC serogroups. Of the other 104 toxin gene positive strains, 80 hybridized with the VT2 probe of which 34 were from cases of porcine post-weaning diarrhoea belonging to serogroup 0138:K81 and 22 were untypable strains from cattle.
使用针对编码热不稳定肠毒素（LTI）、热稳定肠毒素（STIa、STII）、维罗毒素（VT1、VT2）以及黏附素K88（F4）、K99（F5）、F41和987P（F6）的基因的DNA基因探针，检测了873株来自腹泻病例的大肠杆菌分离株（680株来自猪，187株来自牛，6株来自羊）。共有188株毒素基因呈阳性，其中84株属于经典肠毒素型大肠杆菌血清群。在其他104株毒素基因阳性菌株中，80株与VT2探针杂交，其中34株来自猪断奶后腹泻病例，属于0138:K81血清群，22株是来自牛的无法分型的菌株。
