通过分子和血清学方法检测犊牛粪便样本中的产肠毒素K99（F5）和F41 。
Detection of enterotoxigenic K99 (F5) and F41 from fecal sample of calves by molecular and serological methods.
作者信息
Shams Zahra, Tahamtan Yahya, Pourbakhsh Ali, Hosseiny Mohammad H, Kargar Mohammad, Hayati Masoumeh
机构信息
1Azad University, Jahrom, Iran.
2Razi Vaccine and Serum Research Institute, Sanaye Sq., Shiraz, Iran.
出版信息
Comp Clin Path. 2012;21(4):475-478. doi: 10.1007/s00580-010-1122-2. Epub 2010 Nov 3.
Abstract
Enterotoxigenic (ETEC) is one of the major causes of neonatal calf diarrhea. Almost all ETEC bacteria are known to adhere to receptors on the small intestinal epithelium via their fimbriae, (F5 (K99) and F41).This study was undertaken to investigate the phenotypic and genotypic screening of virulence genes in K99 and F41. During January 2008 to December 2009, 298 diarrheic neonatal calves at 1-30 days old were studied by multiplex PCR, isolation, and serological grouping. Of the 298 diarrheic samples, 268 were isolated, of which 16 samples (5.3%) were positive for having the F5 (K99) fimbrial gene by PCR while all of the isolates also carried F41 fimbrial genes. Twenty-five percent of the isolates were proven not to be toxigenic as they did not possess the STa enterotoxin gene.
摘要
产肠毒素大肠杆菌（ETEC）是新生犊牛腹泻的主要病因之一。几乎所有的ETEC细菌都通过其菌毛（F5（K99）和F41）粘附于小肠上皮细胞的受体。本研究旨在对K99和F41毒力基因进行表型和基因型筛选。在2008年1月至2009年12月期间，对298头1至30日龄腹泻新生犊牛进行了多重PCR、分离及血清学分型研究。在298份腹泻样本中，分离出268份，其中16份样本（5.3%）经PCR检测F5（K99）菌毛基因呈阳性，而所有分离株均携带F41菌毛基因。25%的分离株被证实不产毒素，因为它们不具有STa肠毒素基因。
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