Lau Aldrich N K, Ohta Aaron T, Phan Huan L, Hsu Hsan-Yin, Jamshidi Arash, Chiou Pei-Yu, Wu Ming C
Applied Biosystems, Foster City, CA 94404, USA.
Lab Chip. 2009 Oct 21;9(20):2952-7. doi: 10.1039/b907840a. Epub 2009 Jul 22.
Optoelectronic tweezers enables parallel manipulation of individual single cells using optical addressing and optically induced dielectrophoretic force. This provides a useful platform for performing a variety of biological functions, such as cell manipulation, cell sorting, and cell electroporation. However, in order to obtain more reliable cellular manipulation, especially of adherent mammalian cells, antifouling coatings need to be used to avoid non-specific cell adherence. Two antifouling coatings are discussed here, which can reduce the amount of non-specific adherence by as much as a factor of 30.
光电镊子能够利用光学寻址和光诱导介电泳力对单个单细胞进行并行操作。这为执行各种生物学功能提供了一个有用的平台，如细胞操作、细胞分选和细胞电穿孔。然而，为了获得更可靠的细胞操作，特别是对于贴壁哺乳动物细胞，需要使用防污涂层来避免非特异性细胞粘附。这里讨论了两种防污涂层，它们可以将非特异性粘附量减少多达30倍。
