NSF Center for Biophotonics Science and Technology, University of California, Davis, Sacramento, California 95817, USA.
Appl Spectrosc. 2010 Nov;64(11):1308-10. doi: 10.1366/000370210793334972.
We report on the development and characterization of a multifocal laser tweezers Raman spectroscopy (M-LTRS) technique for parallel Raman spectral acquisition of individual biological cells. Using a 785-nm diode laser and a time-sharing laser trapping scheme, multiple laser foci are generated to optically trap single polystyrene beads and suspension cells in a linear pattern. Raman signals from the trapped objects are simultaneously projected through the slit of a spectrometer and spatially resolved on a charge-coupled device (CCD) detector with minimal signal crosstalk between neighboring cells. By improving the rate of single-cell analysis, M-LTRS is expected to be a valuable method for studying single-cell dynamics of cell populations and for the development of high-throughput Raman based cytometers.
我们报告了一种用于单个生物细胞的并行拉曼光谱采集的多焦激光镊子拉曼光谱（M-LTRS）技术的开发和特性。使用 785nm 二极管激光器和分时激光捕获方案，生成多个激光焦点以在线性图案中光学捕获单个聚苯乙烯珠和悬浮细胞。从捕获物体的拉曼信号同时通过光谱仪的狭缝投射，并在电荷耦合器件（CCD）检测器上进行空间分辨，相邻细胞之间的信号串扰最小。通过提高单细胞分析的速度，M-LTRS 有望成为研究细胞群体中单细胞动力学和开发高通量基于拉曼的细胞仪的有价值方法。
