Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Germany.
Biodegradation. 2010 Apr;21(2):309-19. doi: 10.1007/s10532-009-9302-y. Epub 2009 Sep 30.
Industrial wastewater from the production of sulfur containing esters and the resulting products of this synthesis, 2-ethylhexylthioglycolic acid (EHTG) and iso-octylthioglycolic acid (IOTG), were deployed in this study to enrich novel bacterial strains, since no wastewater and EHTG or IOTG degrading microorganisms were hitherto described or available. In addition, nothing is known about the biodegradation of these thiochemicals. The effect of this specific wastewater on the growth behaviour of microorganisms was investigated using three well-known Gram-negative bacteria (Escherichia coli, Pseudomonas putida, and Ralstonia eutropha). Concentrations of 5% (v/v) wastewater in complex media completely inhibited growth of these three bacterial strains. Six bacterial strains were successfully isolated, characterized and identified by sequencing their 16S rRNA genes. Two isolates referred to as Achromobacter sp. strain MT-E3 and Pseudomonas sp. strain MT-I1 used EHTG or IOTG, respectively, as well as the wastewater as sole source of carbon and energy for weak growth. More notably, both isolates removed these sulfur containing esters in remarkable amounts from the cultures supernatant. One further isolate was referred to as Klebsiella sp. strain 58 and exhibited an unusual high tolerance against the wastewater's toxicity without utilizing the contaminative compounds. If cultivated with gluconic acid as additional carbon source, the strain grew even in presence of more than 40% (v/v) wastewater. Three other isolates belonging to the genera Bordetella and Pseudomonas tolerated these organic sulfur compounds but showed no degradation abilities.
本研究利用生产含硫酯的工业废水及该合成的产物 2-乙基己基硫代甘氨酸（EHTG）和异辛基硫代甘氨酸（IOTG）来富集新型细菌菌株，因为迄今为止尚未描述或获得废水和 EHTG 或 IOTG 降解微生物。此外，人们对这些硫化学物质的生物降解知之甚少。使用三种知名的革兰氏阴性菌（大肠杆菌、假单胞菌和罗尔斯顿氏菌）研究了这种特定废水对微生物生长行为的影响。在复杂培养基中，5%（v/v）废水的浓度完全抑制了这三种细菌菌株的生长。成功分离出六株细菌，并通过测序其 16S rRNA 基因对其进行了表征和鉴定。两种分离株分别被称为 Achromobacter sp. 菌株 MT-E3 和 Pseudomonas sp. 菌株 MT-I1，分别使用 EHTG 或 IOTG 以及废水作为唯一的碳源和能源进行微弱生长。值得注意的是，这两种分离株都能从培养物上清液中大量去除这些含硫酯。另一种分离株被称为 Klebsiella sp. 菌株 58，对废水的毒性具有异常高的耐受性，而不利用污染化合物。如果与葡萄糖酸一起培养作为额外的碳源，即使在存在超过 40%（v/v）废水的情况下，该菌株也能生长。另外三个属于博德特氏菌和假单胞菌属的分离株耐受这些有机硫化合物，但没有降解能力。
