Department of Industrial Biotechnology, Universiti Teknologi Malaysia, Johor, Malaysia.
Chemosphere. 2011 Jan;82(4):507-13. doi: 10.1016/j.chemosphere.2010.10.094. Epub 2010 Nov 20.
A co-culture consisting of Hydrogenophaga sp. PBC and Ralstonia sp. PBA, isolated from textile wastewater treatment plant could tolerate up to 100 mM 4-aminobenzenesulfonate (4-ABS) and utilize it as sole carbon, nitrogen and sulfur source under aerobic condition. The biodegradation of 4-ABS resulted in the release of nitrogen and sulfur in the form of ammonium and sulfate respectively. Ninety-eight percent removal of chemical oxygen demand attributed to 20 mM of 4-ABS in cell-free supernatant could be achieved after 118 h. Effective biodegradation of 4-ABS occurred at pH ranging from 6 to 8. During batch culture with 4-ABS as sole carbon and nitrogen source, the ratio of strain PBA to PBC was dynamic and a critical concentration of strain PBA has to be reached in order to enable effective biodegradation of 4-ABS. Haldane inhibition model was used to fit the degradation rate at different initial concentrations and the parameters μ(max), K(s) and K(i) were determined to be 0.13 h⁻¹, 1.3 mM and 42 mM respectively. HPLC analyses revealed traced accumulation of 4-sulfocatechol and at least four unidentified metabolites during biodegradation. This is the first study to report on the characterization of 4-ABS-degrading bacterial consortium that was isolated from textile wastewater treatment plant.
从纺织废水处理厂分离出的氢噬菌属 PBC 和罗尔斯顿氏菌属 PBA 共培养物能够耐受高达 100mM 的对氨基苯磺酸盐（4-ABS），并在有氧条件下将其作为唯一的碳、氮和硫源进行利用。4-ABS 的生物降解导致氮和硫分别以铵盐和硫酸盐的形式释放。在无细胞上清液中添加 20mM 的 4-ABS 后，经过 118 小时，化学需氧量的去除率达到了 98%。在 pH 值为 6 至 8 的范围内，4-ABS 能够有效生物降解。在以 4-ABS 为唯一碳源和氮源的分批培养中，PBA 菌株与 PBC 菌株的比例是动态的，必须达到 PBA 菌株的临界浓度，才能实现 4-ABS 的有效生物降解。使用 Haldane 抑制模型来拟合不同初始浓度下的降解速率，并确定了 μ(max)、K(s)和 K(i)参数分别为 0.13 h⁻¹、1.3mM 和 42mM。HPLC 分析表明，在生物降解过程中， traced accumulation 了 4-磺基邻苯二酚和至少四种未知代谢物。这是首次报道从纺织废水处理厂分离出的 4-ABS 降解细菌共混物的特性研究。
