Kubonishi I, Sonobe H, Miyagi T, Iwahara Y, Ohyashiki J H, Ohyashiki K, Toyama K, Ohtsuki Y, Miyoshi I
Department of Medicine, Kochi Medical School, Japan.
Cancer Res. 1990 Dec 1;50(23):7682-5.
A new human lymphoma cell line, designated DL-40, was established from the peripheral blood of a 64-year-old woman with leukemic conversion of aggressive large cell lymphoma. The cell line grew in suspension with or without forming clumps of cells and exhibited large, round, or multiple nuclei in the relatively abundant cytoplasm that was positive for acid phosphatase. The cells expressed a Ki-1 antigen (CD30), E+, CD2+, CD4+, CD45+, Ia+ phenotype and had rearranged T-cell receptor beta chain but were negative for CD15, HTLV-I, and Epstein-Barr virus nuclear antigen. Chromosome analysis of this cell line showed a human female karyotype with complex hyperdiploid abnormalities. DL-40 cells produced tumors histologically similar to the original lymphoma when transplanted into nude mice and immunosuppressed hamsters. The DL-40 cell line could provide a useful tool for the understanding of biology of the Ki-1-positive non-Hodgkin's lymphoma.
一种新的人类淋巴瘤细胞系，命名为DL-40，是从一名64岁患有侵袭性大细胞淋巴瘤白血病转化的女性外周血中建立的。该细胞系在悬浮培养时可形成或不形成细胞团块生长，在相对丰富的胞质中呈现大的、圆形的或多个细胞核，酸性磷酸酶呈阳性。这些细胞表达Ki-1抗原（CD30）、E+、CD2+、CD4+、CD45+、Ia+表型，T细胞受体β链发生重排，但CD15、HTLV-I和爱泼斯坦-巴尔病毒核抗原呈阴性。对该细胞系的染色体分析显示为具有复杂超二倍体异常的人类女性核型。当将DL-40细胞移植到裸鼠和免疫抑制仓鼠体内时，会产生组织学上与原始淋巴瘤相似的肿瘤。DL-40细胞系可为理解Ki-1阳性非霍奇金淋巴瘤的生物学特性提供有用的工具。
