del Mistro A, Leszl A, Bertorelle R, Calabro M L, Panozzo M, Menin C, D'Andrea E, Chieco-Bianchi L
Istituto di Oncologia, CIRC, Università di Padova, Italy.
Leukemia. 1994 Jul;8(7):1214-9.
Ten months following the diagnosis of Hodgkin's disease (HD), a 46-year-old woman presented cutaneous and leukemic involvement by CD30+ anaplastic large cells, from which a continuously growing, exogenous growth factor-independent T cell line was established. The cultured cells are phenotypically and genotypically T cell in type, negative for EBV, HTLV-I and HTLV-II viral sequences, and release soluble CD30 into the supernatant. Karyotype analysis disclosed several chromosomal abnormalities, but none on chromosome 5q. The involvement of the short arm of chromosome 17 prompted us to investigate the TP53 gene by means of the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) analysis, but no alterations were found in exons 5-8.
在诊断为霍奇金淋巴瘤（HD）十个月后，一名46岁女性出现皮肤和白血病受累，受累细胞为CD30 +间变性大细胞，从中建立了一种持续生长、不依赖外源性生长因子的T细胞系。培养的细胞在表型和基因型上均为T细胞类型，EBV、HTLV-I和HTLV-II病毒序列检测为阴性，并将可溶性CD30释放到上清液中。核型分析发现了几种染色体异常，但5号染色体长臂未发现异常。17号染色体短臂受累促使我们通过聚合酶链反应单链构象多态性（PCR-SSCP）分析来研究TP53基因，但在第5至8外显子中未发现改变。
