Department of Genetics, Dr. A.L.M. Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai, India.
Drug Chem Toxicol. 2009;32(4):411-6. doi: 10.1080/01480540903127316.
The antigenotoxic potential of an aqueous fraction of ethanol extract of Mentha spicata was evaluated by measuring the frequency of micronucleated polychromatic erythrocytes (MnPCEs) in mice bone marrow, using 4-nitroquinoline-1oxide (NQO) as the reference mutagen. In addition, lipid peroxidation (LPO) and antioxidant levels were also quantified with liver tissue of the same mice to assess their antioxidant potential. Swiss albino mice of either sex (25-30 g) were orally pretreated with an aqueous fraction (80, 160, and 320 mg/kg) for 5 consecutive days. NQO (7.5 mg/kg) was injected intraperitoneally after 2 hours until the final day (day 5) of treatment with aqueous fraction. Animals were sacrificed 24 hours later by cervical dislocation and processed for micronuclei and bioassays. A significant reduction (about 67%) of NQO-induced MnPCE frequency was observed at the dose of 320 mg/kg. The LPO was also suppressed effectively, with concomitant changes in both enzymatic and nonenzymatic antioxidants. The restoration level was dose dependent in LPO and glutathione-s-transferase, whereas it was dose independent in superoxide dismutase, glutathione peroxidase, catalase, and reduced glutathione. The results indicate that the aqueous fraction of M. spicata mediates their antigenotoxic effects by the modulation of LPO and antioxidant enzymes.
采用 4-硝基喹啉 1-氧化物（NQO）作为参考诱变剂，评估了薄荷属乙醇提取物水提物的抗原毒性潜力，通过测量小鼠骨髓中具有嗜多染红细胞微核的频率（MnPCEs）来进行评估。此外，还使用相同小鼠的肝组织来定量测定脂质过氧化（LPO）和抗氧化剂水平，以评估其抗氧化潜力。用雌雄两性（25-30g）瑞士白化小鼠，连续 5 天经口给予水提物（80、160 和 320mg/kg）。在给予水提物的最后一天（第 5 天），2 小时后腹膜内注射 NQO（7.5mg/kg）。24 小时后，通过颈椎脱位处死动物，并进行微核和生物测定。在 320mg/kg 剂量下，观察到 NQO 诱导的 MnPCE 频率显著降低（约 67%）。LPO 也得到了有效抑制，同时改变了酶和非酶抗氧化剂。LPO 和谷胱甘肽-S-转移酶的恢复水平呈剂量依赖性，而超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶和还原型谷胱甘肽的恢复水平则呈剂量非依赖性。结果表明，薄荷属水提物通过调节 LPO 和抗氧化酶来介导其抗原毒性作用。
