Department of Genetics, Dr. A.L.M. Post Graduate Institute of Basic Medical Sciences, University of Madras India.
Genet Mol Biol. 2009 Oct;32(4):847-52. doi: 10.1590/S1415-47572009005000086. Epub 2009 Dec 1.
Spearmint leaves (Mentha spicata L.) contain high levels of antioxidants that are known to protect against both exogenous and endogenous DNA damage. In this study, the protective effects of the hexane fraction (HF), chloroform fraction (CF) and ethyl acetate fraction (EAF) in an ethanol extract from M. spicata were evaluated against 4-nitroquinoline-1-oxide (4-NQO) induced chromosome damage and apoptosis in bone marrow cells of Swiss albino mice. Two (EAF; 80 and 160 mg/ kg body weight - bw) or three (HF and CF; 80, 160 and 320 mg/ kg bw) doses of solvent fractions or vehicle control (25% DMSO in water) were administered orally for five consecutive days. Upon the sixth day, 4-NQO was injected intraperitoneally. The animals were killed the following day. Other control groups were comprised of animals treated with either the vehicle control or the various doses of solvent fractions, but with no 4-NQO treatment. 4-NQO induced micro-nucleated polychromatic erythrocytes (MnPCEs) in all the test groups. However, pre-treatment of animals with the solvent fractions significantly reduced the 4-NQO-induced MnPCEs as well as the percentage of apoptotic cells. The reduction of both MnPCE and apoptosis was more evident following the pre-treatment of animals with 160 mg/kg bw EAF.
留兰香叶片（Mentha spicata L.）含有高水平的抗氧化剂，已知可预防外源性和内源性 DNA 损伤。在这项研究中，评估了留兰香乙醇提取物的正己烷部分（HF）、氯仿部分（CF）和乙酸乙酯部分（EAF）对 4-硝基喹啉-1-氧化物（4-NQO）诱导的瑞士白化病小鼠骨髓细胞染色体损伤和细胞凋亡的保护作用。两种（EAF；80 和 160 mg/kg 体重）或三种（HF 和 CF；80、160 和 320 mg/kg bw）溶剂部分剂量或溶剂对照（25% DMSO 在水中）连续口服 5 天。第六天，腹腔内注射 4-NQO。第二天处死动物。其他对照组由用溶剂对照或各种剂量的溶剂部分处理但未用 4-NQO 处理的动物组成。4-NQO 诱导所有测试组的微核多色红细胞（MnPCEs）。然而，用溶剂部分预先处理动物可显著降低 4-NQO 诱导的 MnPCE 以及凋亡细胞的百分比。在用 160 mg/kg bw EAF 预处理动物后，MnPCE 和细胞凋亡的减少更为明显。
