通过等离子体聚合将 DNA 固定在微孔 PVDF 膜上。

Immobilization of DNA on microporous PVDF membranes by plasma polymerization.

机构信息

Department of Chemical and Materials Engineering, Tamkang University, Taipei 25137, Taiwan.

出版信息

J Biomater Sci Polym Ed. 2009;20(13):1943-59. doi: 10.1163/156856208X396047.

PMID:19793449

Abstract

Microporous poly(vinylidene fluoride) (PVDF) membranes with different porous surface morphologies were prepared by immersion-precipitation from coagulation baths of different strengths. On these membranes single-strand deoxyribonucleic acid (ss-DNA) was covalently immobilized by a dual-step procedure. First, poly(glycidyl methacrylate) (PGMA) was grafted on PVDF membranes by plasma-induced free radical polymerization. Then, ss-DNA was bonded to PGMA through ring-opening reactions of epoxies with amine to form amino alcohols. The highest attainable graft yield of PGMA on PVDF membrane was 0.3 mg/cm(2), which was the case when a highly porous PVDF membrane was employed as the substrate. For immobilization of ss-DNA, the yield was found to depend significantly on the reaction temperature and pH. The maximal value was 48.5 mug/cm(2). Furthermore, adsorption tests of anti-DNA antibody were carried out on membranes with and without immobilized ss-DNA using serum obtained from systemic lupus erythematosus patients. The results indicated that the immobilized DNA could effectively adsorb the antibody in the serum.

摘要

采用不同浓度的凝固浴通过浸没沉淀法制得具有不同多孔表面形貌的微孔聚偏二氟乙烯（PVDF）膜。在这些膜上，通过两步法共价固定单链脱氧核糖核酸（ss-DNA）。首先，通过等离子体引发的自由基聚合将聚（甲基丙烯酸缩水甘油酯）（PGMA）接枝到 PVDF 膜上。然后，通过环氧与胺的开环反应将 ss-DNA 键合到 PGMA 上，形成氨基醇。当使用高多孔性的 PVDF 膜作为基底时，PGMA 在 PVDF 膜上的最大接枝收率为 0.3mg/cm(2)。对于 ss-DNA 的固定，发现收率明显取决于反应温度和 pH 值。最大值为 48.5µg/cm(2)。此外，用来自系统性红斑狼疮患者的血清对固定有和未固定 ss-DNA 的膜进行了抗-DNA 抗体的吸附试验。结果表明，固定的 DNA 可以有效地吸附血清中的抗体。