Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
J Gen Virol. 2010 Jan;91(Pt 1):294-305. doi: 10.1099/vir.0.015990-0. Epub 2009 Sep 30.
Rice stripe disease, caused by rice stripe virus (RSV), is one of the major virus diseases in east Asia. Rice plants infected with RSV usually show symptoms such as chlorosis, weakness, necrosis in newly emerged leaves and stunting. To reveal rice cellular systems influenced by RSV infection, temporal changes in the transcriptome of RSV-infected plants were monitored by a customized rice oligoarray system. The transcriptome changes in RSV-infected plants indicated that protein-synthesis machineries and energy production in the mitochondrion were activated by RSV infection, whereas energy production in the chloroplast and synthesis of cell-structure components were suppressed. The transcription of genes related to host-defence systems under hormone signals and those for gene silencing were not activated at the early infection phase. Together with concurrent observation of virus concentration and symptom development, such transcriptome changes in RSV-infected plants suggest that different sets of various host genes are regulated depending on the development of disease symptoms and the accumulation of RSV.
水稻条纹病毒病（Rice stripe disease），又称水稻条斑病，是东亚地区主要的病毒病之一。受水稻条纹病毒（RSV）感染的水稻植株通常表现出黄化、衰弱、新叶坏死和矮化等症状。为了揭示受 RSV 感染影响的水稻细胞系统，采用定制的水稻寡核苷酸芯片系统监测 RSV 感染植物的转录组的时间变化。受 RSV 感染的水稻植物的转录组变化表明，蛋白质合成机制和线粒体的能量产生被 RSV 感染激活，而叶绿体的能量产生和细胞结构成分的合成受到抑制。在激素信号下与宿主防御系统相关的基因以及参与基因沉默的基因的转录在早期感染阶段没有被激活。与病毒浓度和症状发展的同时观察表明，受 RSV 感染的植物的这些转录组变化表明，不同的宿主基因集根据疾病症状的发展和 RSV 的积累而受到不同的调控。
