Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110021, India.
Arch Virol. 2021 May;166(5):1325-1336. doi: 10.1007/s00705-021-05006-0. Epub 2021 Mar 3.
Rice tungro disease (RTD) is a devastating disease of rice caused by combined infection with rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV), with one of the main symptoms being stunting. To dissect the molecular events responsible for RTD-induced stunting, the expression patterns of 23 cell-wall-related genes were examined in different rice lines with the same titers of RTSV but different titers of RTBV and in lines where only RTBV was present. Genes encoding cellulose synthases, expansins, glycosyl hydrolases, exostosins, and xyloglucan galactosyl transferase showed downregulation, whereas those encoding defensin or defensin-like proteins showed upregulation with increasing titers of RTBV. RTSV titers did not affect the expression levels of these genes. A similar relationship was seen for the reduction in the cellulose and pectin content and the accumulation of lignin. In silico analysis of promoters of the genes indicated a possible link to transcription factors reported earlier to respond to viral titers in rice. These results suggest a common network in which the genes related to the cell wall components are affected during infection with diverse viruses in rice.
水稻矮缩病（RTD）是一种严重危害水稻的疾病，由水稻曲叶病毒（RTBV）和水稻曲叶球形病毒（RTSV）的联合感染引起，其主要症状之一是植株矮化。为了剖析导致 RTD 诱导矮化的分子事件，在具有相同 RTSV 滴度但 RTBV 滴度不同的不同水稻品系中，以及在仅存在 RTBV 的品系中，检测了 23 个细胞壁相关基因的表达模式。纤维素合酶、扩展蛋白、糖苷水解酶、外切素和木葡聚糖半乳糖基转移酶的编码基因表达下调，而随着 RTBV 滴度的增加，防御素或防御素样蛋白的编码基因表达上调。RTSV 滴度不影响这些基因的表达水平。类似的关系也存在于纤维素和果胶含量的降低和木质素的积累中。对这些基因启动子的计算机分析表明，与之前报道的水稻中响应病毒滴度的转录因子可能存在联系。这些结果表明，在水稻中感染不同病毒时，与细胞壁成分相关的基因受到影响，存在一个共同的网络。
