Gracie J A, Sarawar S R, Bolton E M, Bradley J A, Tanaka T, Bell E B
University Department of Surgery, Western Infirmary, Glasgow, Scotland.
Transplantation. 1990 Dec;50(6):996-1000. doi: 10.1097/00007890-199012000-00020.
PVG-rnu/rnu nude rats reject a fully allogeneic DA renal allograft after the adoptive transfer of naive CD4+ T cells alone, but rejection is accompanied by the accumulation of many CD8+ leukocytes within the graft. In order to clearly establish the provenance of these CD8+ cells infiltrating rejecting kidney allografts, nude recipients (PVG-RT7a) were injected with CD4+ T cells from the PVG-RT7b congenic strain bearing an allotypic variant of the leukocyte-common antigen. Dual fluorescence and immunohistochemistry demonstrated that approximately 75% of the total infiltrate was host-derived; the donor-derived RT7b population was almost entirely (92-99%) CD4+, CD5+, CD3+, and alpha beta TCR+. At least 97% of the CD8+ cells were of nude origin. There was no evidence of donor-derived CD8+ cells or of a CD4+8+ double-staining population. Unexpectedly, nearly half of the alpha beta TCR+ cells from the grafts were of nude origin.
PVG-rnu/rnu裸鼠在单独过继转移幼稚CD4⁺T细胞后会排斥完全同种异体的DA肾移植，但排斥反应伴随着移植物内许多CD8⁺白细胞的积累。为了明确浸润排斥性肾移植的这些CD8⁺细胞的来源，给裸鼠受体（PVG-RT7a）注射来自携带白细胞共同抗原异型变体的PVG-RT7b同基因品系的CD4⁺T细胞。双重荧光和免疫组织化学显示，总浸润细胞中约75%来自宿主；供体来源的RT7b群体几乎全部（92-99%）为CD4⁺、CD5⁺、CD3⁺和αβTCR⁺。至少97%的CD8⁺细胞来源于裸鼠。没有供体来源的CD8⁺细胞或CD4⁺8⁺双染群体的证据。出乎意料的是，移植物中近一半的αβTCR⁺细胞来源于裸鼠。
