Systems Toxicology Unit, Institute for Health and Consumer Protection, European Commission-DG Joint Research Centre, I-21027 Ispra (VA), Italy.
Opt Lett. 2009 Oct 1;34(19):3006-8. doi: 10.1364/OL.34.003006.
A holographic optical lithography setup with extremely small longitudinal spherical aberration is described. The setup is used for the fabrication of holographic diffractive optical elements intended to collect light emitted from a fluorescence spot located on a biochip surface. A key feature of the optical setup is its ability to simulate a point-source-like fluorescence spot. A detailed description of the setup together with its optical properties are provided. The fluorescence light collection efficiency of the holographic diffractive optical elements produced using this setup is demonstrated.
介绍了一种具有极小纵向球差的全息光学光刻系统。该系统用于制作全息衍射光学元件，用于收集位于生物芯片表面上的荧光点发出的光。光学系统的一个关键特点是能够模拟点源样的荧光点。提供了该系统的详细描述及其光学特性。展示了使用该系统制作的全息衍射光学元件的荧光光收集效率。
