Macko Peter, Whelan Maurice P
Nanotechnology and Molecular Imaging Unit, Institute for Health and Consumer Protection, European Commission-DG Joint Research Centre, Ispra, Italy.
Opt Lett. 2008 Nov 15;33(22):2614-6. doi: 10.1364/ol.33.002614.
An approach to enhancing the light collection from fluorescence emitters bound on the surface of a biochip is presented. It is based on the integration of diffractive optical elements on the underside of the chip that are essentially thick volume holograms written into a layer of photopolymer recording material. The high diffractive efficiency and angular selectivity of these types of diffractive elements make them very effective collectors of the spatially anisotropic light emitted by surface-bound fluorophores. The holographic lithography setup used to fabricate the diffractive elements is described. Results obtained using both a focused laser and light from a fluorescence spot to characterize the performance of the diffractive optical elements are presented.
本文提出了一种增强从生物芯片表面结合的荧光发射体收集光的方法。该方法基于在芯片底部集成衍射光学元件，这些元件本质上是写入光聚合物记录材料层中的厚体全息图。这类衍射元件的高衍射效率和角度选择性使其成为表面结合荧光团发射的空间各向异性光的非常有效的收集器。描述了用于制造衍射元件的全息光刻装置。展示了使用聚焦激光和来自荧光点的光来表征衍射光学元件性能所获得的结果。
