de Abin Martha Fuentes, Spronk Gordon, Wagner Mark, Fitzsimmons Mark, Abrahante Juan E, Murtaugh Michael P
Department of Veterinary and Biomedical Sciences, University of Minnesota, St Paul, MN 55108, USA.
Can J Vet Res. 2009 Jul;73(3):200-4.
Isolation of Porcine reproductive and respiratory syndrome virus (PRRSV) on MA104 or MARC-145 cells is frequently used in PRRS diagnosis. However, the ability of recent field isolates to grow on these established simian cell lines has not been determined. The aim of this study was to characterize the growth of PRRSV field isolates on primary porcine alveolar macrophages (PAMs) and MA104 cells in comparison with the growth of the laboratory-adapted strain VR-2332. A cytopathic effect was observed in 70% of serum samples after 1 passage on PAMs and was verified by immunofluorescent staining or reverse transcriptase-polymerase chain reaction. Field isolate growth was observed on MA104 cells for only 1 of 50 serum samples after 14 d. Strain VR-2332 grew readily in MA104 cells [maximum titer, 10(7) TCID(50) (median tissue culture infective dose) per milliliter at 30 h] but not in PAMs (10(2) TCID(50)/mL at 72 h). These results show that PAMs are superior to simian cells for diagnostic isolation of current field PRRSV strains.
在PRRS诊断中，常在MA104或MARC - 145细胞上分离猪繁殖与呼吸综合征病毒（PRRSV）。然而，近期的田间分离株在这些已建立的猴细胞系上生长的能力尚未确定。本研究的目的是将PRRSV田间分离株在原代猪肺泡巨噬细胞（PAM）和MA104细胞上的生长特性与实验室适应株VR - 2332的生长情况进行比较。在PAM上传代1次后，70%的血清样本中观察到细胞病变效应，并通过免疫荧光染色或逆转录 - 聚合酶链反应进行了验证。在MA104细胞上，50份血清样本中只有1份在14天后观察到田间分离株生长。VR - 2332株在MA104细胞中易于生长[30小时时的最大滴度为每毫升10(7) TCID(50)（半数组织培养感染剂量）]，但在PAM中则不然（72小时时为10(2) TCID(50)/mL）。这些结果表明，对于当前田间PRRSV株的诊断性分离，PAM优于猴细胞。
