Xu Hu, Gong Bangjun, Sun Qi, Li Chao, Zhao Jing, Xiang Lirun, Li Wansheng, Guo Zhenyang, Tang Yan-Dong, Leng Chaoliang, Li Zhen, Wang Qian, Zhou Guohui, An Tongqing, Cai Xuehui, Tian Zhi-Jun, Peng Jinmei, Zhang Hongliang
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Henan Key Laboratory of Insect Biology in Funiu Mountain, Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang 473061, China.
Transbound Emerg Dis. 2023 Mar 27;2023:6793604. doi: 10.1155/2023/6793604. eCollection 2023.
Porcine reproductive and respiratory syndrome virus (PRRSV)-1 and PRRSV-2 have long been cocirculating in China. To date, all PRRSV-1 strains in China have been classified as subtype 1. We investigated the prevalence of PRRSV-1 in several areas of China from 2016 to 2022 and found that BJEU06-1-like strains comprised the main epidemic branch of PRRSV-1. Pathogenicity data for this subgroup are currently lacking. In this study, the Chinese BJEU06-1-like PRRSV-1 strain ZD-1 was isolated from primary alveolar macrophages (PAMs). ZD-1 has undergone no recombination and has a 5-aa discontinuous deletion in the Nsp2 protein, similar to other BJEU06-1-like strains; additionally, ZD-1 has a 26 aa C-terminal truncation in the GP3 gene. Pathogenicity studies revealed that ZD-1 causes obvious clinical symptoms: prolonged fever; reduced body weight; alveolar epithelial proliferation and moderate alveolar diaphragm widening in the lungs; diffuse lymphocytic hyperplasia in the lymph nodes; high levels of viremia in the serum; and elevated viral loads in the lungs, lymph nodes, and tonsils. These results suggested that the BJEU06-1-like PRRSV-1 strain ZD-1 is moderately pathogenic to piglets. This is the first study to evaluate the pathogenicity of the BJEU06-1-like branch in China, enriching the understanding of PRRSV-1 in China.
猪繁殖与呼吸综合征病毒（PRRSV）-1和PRRSV-2长期以来在中国共同流行。迄今为止，中国所有的PRRSV-1毒株都被归类为1型亚型。我们调查了2016年至2022年中国几个地区PRRSV-1的流行情况，发现BJEU06-1样毒株构成了PRRSV-1的主要流行分支。目前缺乏该亚组的致病性数据。在本研究中，中国BJEU06-1样PRRSV-1毒株ZD-1从原代肺泡巨噬细胞（PAM）中分离得到。ZD-1未发生重组，在Nsp2蛋白中有一个5个氨基酸的不连续缺失，这与其他BJEU06-1样毒株相似；此外，ZD-1在GP3基因中有一个26个氨基酸的C末端截短。致病性研究表明，ZD-1会引起明显的临床症状：持续发热；体重减轻；肺部肺泡上皮细胞增殖和肺泡隔中度增宽；淋巴结弥漫性淋巴细胞增生；血清中病毒血症水平高；以及肺、淋巴结和扁桃体中病毒载量升高。这些结果表明，BJEU06-1样PRRSV-1毒株ZD-1对仔猪具有中等致病性。这是首次评估中国BJEU06-1样分支致病性的研究，丰富了对中国PRRSV-1的认识。
