Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, Limassol, Cyprus.
Plant Signal Behav. 2012 Mar;7(3):342-4. doi: 10.4161/psb.19236. Epub 2012 Mar 1.
Recent years have seen a remarkable increase in the number of publications dealing with the application of epifluorescence microscopy in cell biology. This can be widely attributed to the development of state-of-the-art image processing programs, as well as the development of new reagents/probes, which allow the labeling of most cell structures, organelles and metabolites with high specificity. However, the use of a specific fluorescent dye, 3,3'-dihexyloxacarbocyanine iodide (DiOC₆), has been recently revisited and several new application potentials have emerged. The goal of this mini-review is to provide an up-to-date overview of the multiple roles of this multifaceted probe.
近年来，应用荧光显微镜于细胞生物学的研究文献数量显著增加。这主要归因于先进的图像处理程序的发展，以及新型试剂/探针的开发，这些试剂/探针使大多数细胞结构、细胞器和代谢物的标记具有高度特异性。然而，最近人们重新审视了一种特定的荧光染料，3,3'-二己氧基羰花青碘化物（DiOC₆），并出现了一些新的应用潜力。本篇综述的目的是提供该多功能探针的多种作用的最新概述。
