Crisan Mihaela, Chen Chien-Wen, Corselli Mirko, Andriolo Gabriella, Lazzari Lorenza, Péault Bruno
Stem Cell Research Center, Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Ann N Y Acad Sci. 2009 Sep;1176:118-23. doi: 10.1111/j.1749-6632.2009.04967.x.
We have identified vascular pericytes in multiple human organs on expression of CD146, NG2, PDGF-Rbeta, and mesenchymal stem cell markers (CD44, CD73, CD90, CD105) and absence of blood, endothelial, and myogenic cell markers. Pericytes purified from all tissues were myogenic in culture and in vivo, sustained long-term culture during which they expressed markers of mesenchymal stem cells, and exhibited, at the clonal level, osteogenic, chondrogenic, and adipogenic potentials. These results suggest that human capillary and microvessel walls all over the organism harbor a reserve of progenitor cells that are at the origin of the elusive mesenchymal stem cells, so far identified only retrospectively in primary tissue cultures.
我们通过CD146、NG2、血小板衍生生长因子受体β（PDGF-Rβ）以及间充质干细胞标志物（CD44、CD73、CD90、CD105）的表达，且不存在血液、内皮及肌源性细胞标志物，在多个人体器官中鉴定出了血管周细胞。从所有组织中纯化出的周细胞在体外培养和体内均具有肌源性，能够维持长期培养，在此期间它们表达间充质干细胞标志物，并在克隆水平上表现出成骨、成软骨及成脂潜能。这些结果表明，遍布全身的人体毛细血管和微血管壁都蕴藏着祖细胞储备，这些祖细胞是难以捉摸的间充质干细胞的来源，迄今为止仅在原代组织培养中通过回顾性研究得以鉴定。
