Zuba-Surma Ewa K, Kucia Magda, Rui Liu, Shin Dong-Myung, Wojakowski Wojtek, Ratajczak Janina, Ratajczak Mariusz Z
Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA.
Ann N Y Acad Sci. 2009 Sep;1176:205-18. doi: 10.1111/j.1749-6632.2009.04562.x.
Fetal liver (FL) has been described as a source of both hematopoietic and nonhematopoietic stem cells. Recently we have purified from murine adult bone marrow (BM) a population of CXCR4(+)Oct-4(+)SSEA-1(+)Sca-1(+)Lin(-)CD45(-) very small embryonic/epiblast-like stem cells (VSELs). By employing several complementary imaging and molecular strategies, we report in this study that VSELs, like hematopoietic stem cells (HSCs), are highly enriched in murine FL during the second trimester of gestation. Subsequently, at the beginning of the third trimester of gestation their number decreases, which corresponds to the time when HSCs egress FL and follow the stromal derived factor-1 (SDF-1) gradient in order to colonize developing BM. Thus, our data support the hypothesis that VSELs are a mobile pool of primitive stem cells that respond to similar chemotactic gradients as HSCs and follow their developmental migratory route.
胎儿肝脏（FL）已被描述为造血干细胞和非造血干细胞的来源。最近，我们从成年小鼠骨髓（BM）中纯化出一群CXCR4(+)Oct-4(+)SSEA-1(+)Sca-1(+)Lin(-)CD45(-)的非常小的胚胎/外胚层样干细胞（VSELs）。通过采用多种互补的成像和分子策略，我们在本研究中报告，在妊娠中期，VSELs与造血干细胞（HSCs）一样，在小鼠FL中高度富集。随后，在妊娠晚期开始时，它们的数量减少，这与HSCs离开FL并沿着基质衍生因子-1（SDF-1）梯度定植到发育中的BM的时间相对应。因此，我们的数据支持这样的假设，即VSELs是一群原始干细胞，它们对与HSCs相似的趋化梯度做出反应，并遵循其发育迁移途径。
