Immune Disease Institute and Program in Cellular and Molecular Medicine, Children's Hospital Boston and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA.
J Histochem Cytochem. 2012 Oct;60(10):723-33. doi: 10.1369/0022155412453052. Epub 2012 Jun 27.
Imaging flow cytometry (IFC) platforms combine features of flow cytometry and fluorescent microscopy with advances in data-processing algorithms. IFC allows multiparametric fluorescent and morphological analysis of thousands of cellular events and has the unique capability of identifying collected events by their real images. IFC allows the analysis of heterogeneous cell populations, where one of the cellular components has low expression (<0.03%) and can be described by Poisson distribution. With the help of IFC, one can address a critical question of statistical analysis of subcellular distribution of proteins in a cell. Here the authors review advantages of IFC in comparison with more traditional technologies, such as Western blotting and flow cytometry (FC), as well as new high-throughput fluorescent microscopy (HTFM), and discuss further developments of this novel analytical technique.
成像流式细胞术（IFC）平台结合了流式细胞术和荧光显微镜的特点，并结合了数据处理算法的进步。IFC 允许对数千个细胞事件进行多参数荧光和形态分析，并且具有通过其真实图像识别已采集事件的独特功能。IFC 允许对异质细胞群体进行分析，其中一个细胞成分的表达水平较低（<0.03%），并且可以用泊松分布来描述。借助 IFC，可以解决细胞内蛋白质亚细胞分布的统计分析中的一个关键问题。在这里，作者综述了 IFC 相对于更传统的技术（例如 Western blot 和流式细胞术（FC））以及新型高通量荧光显微镜（HTFM）的优势，并讨论了该新型分析技术的进一步发展。
