Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea.
Clin Exp Rheumatol. 2009 Mar-Apr;27(2 Suppl 53):S63-6.
alpha-Enolase is a target antigen of IgM-type anti-endothelial cell antibody in patients with Behçet's disease (BD). The objective of this study was to assess the reactivity of serum anti-alpha-enolase antibodies in BD and in other rheumatologic diseases, and to evaluate the clinical significance of serum anti-alpha-enolase antibodies in BD.
Enzyme-linked immunosorbent assay (ELISA) and immunoblotting were used to examine serum samples from patients with BD (n=100), systemic lupus erythematosus (SLE) (n=50), systemic sclerosis (n=21), rheumatoid arthritis (RA) (n=20), Takayasu's arteritis (n=20), dermatomyositis (n=17), mixed connective tissue disease (MCTD) (n=11), and samples from healthy volunteer donors (n=23). The medical records of patients with BD were reviewed to investigate their clinical characteristics.
Specific positive signals against recombinant human alpha-enolase were detected by IgM ELISA of serum samples from 56 of the 100 BD patients (56.0%), 24 of the 50 SLE patients (48.0%), 15 of the 21 systemic sclerosis patients (71.4%), 13 of the 20 RA patients (65.0%), 10 of the 20 Takayasu's arteritis patients (50.0%), 9 of the 17 dermatomyositis patients (52.9%), and 5 of the 11 MCTD patients (45.5%). The number of BD patients with vascular lesions was significantly higher in the anti-alpha-enolase antibody positive group than in the negative group (p=0.027).
We demonstrated the reactivities of serum anti-alpha-enolase antibodies in BD and other rheumatologic diseases with moderate specificity and also found that serum anti-alpha-enolase antibodies in BD can be associated with vascular system involvement.
α-烯醇化酶是贝赫切特病（BD）患者 IgM 型抗内皮细胞抗体的靶抗原。本研究旨在评估血清抗α-烯醇化酶抗体在 BD 及其他风湿性疾病中的反应性，并评价血清抗α-烯醇化酶抗体在 BD 中的临床意义。
采用酶联免疫吸附试验（ELISA）和免疫印迹法检测 100 例 BD 患者、50 例系统性红斑狼疮（SLE）患者、21 例系统性硬皮病患者、20 例类风湿关节炎（RA）患者、20 例大动脉炎患者、17 例皮肌炎患者、11 例混合性结缔组织病患者及 23 例健康志愿者的血清样本。回顾性分析 BD 患者的临床资料。
在 100 例 BD 患者中，56 例（56.0%）血清样本的 IgM ELISA 对重组人α-烯醇化酶呈特异性阳性信号，在 50 例 SLE 患者中，24 例（48.0%）呈阳性，在 21 例系统性硬皮病患者中，15 例（71.4%）呈阳性，在 20 例 RA 患者中，13 例（65.0%）呈阳性，在 20 例大动脉炎患者中，10 例（50.0%）呈阳性，在 17 例皮肌炎患者中，9 例（52.9%）呈阳性，在 11 例混合性结缔组织病患者中，5 例（45.5%）呈阳性。抗α-烯醇化酶抗体阳性组 BD 患者血管病变的发生率明显高于阴性组（p=0.027）。
我们证实了血清抗α-烯醇化酶抗体在 BD 及其他风湿性疾病中的反应性，且具有中等特异性，并发现 BD 患者血清抗α-烯醇化酶抗体与血管系统受累有关。
