白塞病患者血清中抗α-烯醇化酶IgG抗体水平升高，且与口腔溃疡的严重程度、红细胞沉降率及C反应蛋白相关。

Serum levels of IgG antibodies against alpha-enolase are increased in patients with Behçet's disease and are associated with the severity of oral ulcer, erythrocyte sedimentation rates, and C-reactive protein.

作者信息

Kang Shin Eui, Lee Sang Jin, Lee Joo Youn, Yoo Hyun Jung, Park Jin Kyun, Lee Eun Young, Lee Eun Bong, Song Young Wook

机构信息

Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Medical Research Centre, Seoul National University, Seoul, Korea.

Dept. of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Medical Research Centre; and Division of Rheumatology, Dept. of Internal Medicine, Seoul National University Hospital, Seoul, Korea.

出版信息

Clin Exp Rheumatol. 2017 Nov-Dec;35 Suppl 108(6):67-74. Epub 2017 Jul 6.

PMID:28770703

Abstract

OBJECTIVES

Behçet's disease (BD) is a chronic inflammatory disease of unknown etiology, characterised by recurrent oral and genital ulcers, skin lesions, uveitis, and arthritis. It is regarded as vasculitis and anti-endothelial cell antibodies (AECA) are found in patients with BD. One of the endothelial cell antibodies was reported to recognise alpha-enolase. This study aimed to investigate expression of alpha-enolase in the surface of peripheral blood cells and serum anti-alpha-enolase antibody (AEA), and their association with clinical manifestations or disease activity of BD.

METHODS

Cell surface alpha-enolase expression was examined from several cell types of peripheral blood, including lymphocytes, monocytes, and neutrophils using flow cytometry in patients with BD and healthy controls (HCs). IgG AEA levels were measured by enzyme-linked immunosorbent assay (ELISA) in sera from 110 patients with BD, and age/sex matched 110 HCs. Association of alpha-enolase or AEA with clinical manifestation was analysed.

RESULTS

The frequency of surface alpha-enolase-expressing cells was increased in BD in lymphocytes and monocytes. Serum AEA levels were in- creased in BD patients (median [IQR], 0.360 [0.268-0.482], p < 0.0001), particularly with mucocutaneous involvement (0.367 [0.273-0.490], p < 0.0001) compared to HCs (0.274 [0.231-0.357]). The levels of AEA were correlated with the number of oral ulcer, ESR, and CRP. There was no association between serum levels of AEA and other clinical manifestations.

CONCLUSIONS

Serum AEA was increased in BD patients and correlated with oral ulcer, ESR and CRP.

摘要

目的

白塞病（BD）是一种病因不明的慢性炎症性疾病，其特征为复发性口腔和生殖器溃疡、皮肤病变、葡萄膜炎和关节炎。它被视为血管炎，且在BD患者中发现了抗内皮细胞抗体（AECA）。据报道，其中一种内皮细胞抗体可识别α-烯醇化酶。本研究旨在调查外周血细胞表面α-烯醇化酶的表达及血清抗α-烯醇化酶抗体（AEA），以及它们与BD临床表现或疾病活动度的关联。

方法

采用流式细胞术检测BD患者和健康对照（HCs）外周血中多种细胞类型（包括淋巴细胞、单核细胞和中性粒细胞）表面α-烯醇化酶的表达。采用酶联免疫吸附测定（ELISA）法检测110例BD患者及年龄/性别匹配的110例HCs血清中的IgG AEA水平。分析α-烯醇化酶或AEA与临床表现的关联。

结果

BD患者淋巴细胞和单核细胞表面表达α-烯醇化酶的细胞频率增加。BD患者血清AEA水平升高（中位数[四分位间距]，0.360[0.268 - 0.482]，p < 0.0001），尤其是与皮肤黏膜受累患者相比（0.367[0.273 - 0.490]，p < 0.0001），而HCs为0.274[0.231 - 0.357]。AEA水平与口腔溃疡数量、血沉（ESR）和C反应蛋白（CRP）相关。AEA血清水平与其他临床表现之间无关联。