Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-871, Republic of Korea.
Plant Physiol Biochem. 2009 Nov-Dec;47(11-12):998-1002. doi: 10.1016/j.plaphy.2009.08.001. Epub 2009 Aug 13.
To elucidate the exact function of CabAS in Centella asiatica, which was previously reported as a putative beta-amyrin synthase [Plant Cell Rep, 24:304-311, 2005], this gene was functionally expressed in the lanosterol synthase-deficient yeast mutant (erg7). After inducing the CabAS gene with galactose, a peak consistent with the dammarenediol standard was detected in LC/APCIMS analyses and the accumulated product was confirmed as dammarenediol. CabAS should therefore be renamed to C. asiatica dammarenediol synthase (CaDDS). The confirmation of this gene function may allow us to better understand the generation of numerous triterpene carbon skeletons.
为了阐明之前被报道为β-香树脂醇合酶的拟南芥角鲨烯合酶（CabAS）在积雪草中的确切功能[植物细胞报告，24：304-311，2005]，本研究在羊毛甾醇合酶缺陷型酵母突变体（erg7）中对该基因进行了功能表达。用半乳糖诱导 CabAS 基因后，在 LC/APCIMS 分析中检测到与达玛烯二醇标准品一致的峰，并且积累的产物被确认为达玛烯二醇。因此，CabAS 应更名为积雪草酸二烯醇合酶（CaDDS）。该基因功能的确认可能使我们能够更好地理解众多三萜碳骨架的生成。
