Loc Nguyen Hoang, Giang Nguyen Thanh, Huy Nguyen Duc
College of Sciences, Hue University, Hue, 530000, Vietnam.
Institute of Biotechnology, Hue University, Hue, 530000, Vietnam.
3 Biotech. 2016 Jun;6(1):86. doi: 10.1007/s13205-016-0404-z. Epub 2016 Mar 5.
In this study, we report the expression level of CaSQS, CabAS and CaCYS, the genes involved in phytosterol and triterpene metabolic pathway of centella (Centella asiatica (L.) Urban), in cells elicited with salicylic acid (50-200 µM). Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis indicated CaSQS, CabAS, and CaCYS genes expressed in both the wild-type and cultured cells (with and without elicitation). In elicited cells, expressions of CaSQS, CabAS, and CaCYS genes showed strong dependence on salicylic acid concentration and elicitation day. The highest expression of CabAS gene was found in the cells elicited with 100 µM salicylic acid on day 10 of inoculation. Salicylic acid treatment (50-200 µM) decreased expression level of CaCYS and CaSQS genes in elicited cells compared with the control.
在本研究中，我们报道了积雪草（Centella asiatica (L.) Urban）植物甾醇和三萜代谢途径中相关基因CaSQS、CabAS和CaCYS在水杨酸（50 - 200 µM）诱导的细胞中的表达水平。逆转录聚合酶链反应（RT-PCR）和Northern印迹分析表明，CaSQS、CabAS和CaCYS基因在野生型细胞和培养细胞（诱导和未诱导）中均有表达。在诱导细胞中，CaSQS、CabAS和CaCYS基因的表达表现出对水杨酸浓度和诱导天数的强烈依赖性。接种第10天，在100 µM水杨酸诱导的细胞中发现CabAS基因的表达最高。与对照相比，水杨酸处理（50 - 200 µM）降低了诱导细胞中CaCYS和CaSQS基因的表达水平。
