Privalsky M L, Sharif M, Yamamoto K R
Department of Microbiology, University of California, Davis 95616.
Cell. 1990 Dec 21;63(6):1277-86. doi: 10.1016/0092-8674(90)90423-c.
The v-erbA oncogene is a retrovirus-transduced and altered copy of a cellular gene for a thyroid hormone receptor. In animal cells, the v-erbA protein fails to respond to hormone and acts as a dominant negative allele, inhibiting gene activation normally conferred by the wild-type thyroid hormone receptor. We report here that, unexpectedly, the v-erbA protein acts as a hormone-regulated transcriptional activator in S. cerevisiae. We suggest that the ability of v-erbA protein to function as a transcriptional repressor or an activator is determined by interaction with, or modification by, other cellular factors, and that this phenomenon may be relevant to understanding ligand regulation of the normal thyroid and steroid hormone receptors.
v-erbA癌基因是一种逆转录病毒转导并改变的细胞甲状腺激素受体基因拷贝。在动物细胞中，v-erbA蛋白对激素无反应，并作为显性负等位基因发挥作用，抑制野生型甲状腺激素受体通常赋予的基因激活。我们在此报告，出乎意料的是，v-erbA蛋白在酿酒酵母中作为激素调节的转录激活因子发挥作用。我们认为，v-erbA蛋白作为转录抑制因子或激活因子发挥功能的能力是由与其他细胞因子的相互作用或修饰所决定的，并且这种现象可能与理解正常甲状腺和类固醇激素受体的配体调节有关。
