Washio K, Ueda S, Misawa S
Department of Legal Medicine, University of Tsukuba, Ibaraki, Japan.
J Forensic Sci. 1990 Nov;35(6):1277-83.
The effects of endogenous 5-methylcytosines on deoxyribonucleic acid (DNA) fingerprints were studied. Analysis with methylation-sensitive restriction endonuclease Sau3AI and its methylation-insensitive isoschizomer MboI showed some differences in the patterns generated as a result of 5-methylcytosines at the recognition sites. Moreover, a few bands of sperm DNA did not match those of blood DNA from the same individual, a phenomenon only observed in the digests of methylation-sensitive endonucleases. These findings indicate the unsuitability of methylation-sensitive restriction endonucleases for DNA fingerprinting and other forms of DNA typing, because of the tissue-specific status of the methylation.
研究了内源性5-甲基胞嘧啶对脱氧核糖核酸（DNA）指纹图谱的影响。使用甲基化敏感限制性内切酶Sau3AI及其甲基化不敏感同裂酶MboI进行分析，结果显示在识别位点处由于5-甲基胞嘧啶而产生的图谱存在一些差异。此外，同一个体的精子DNA的一些条带与血液DNA的条带不匹配，这种现象仅在甲基化敏感内切酶的消化产物中观察到。这些发现表明，由于甲基化的组织特异性状态，甲基化敏感限制性内切酶不适用于DNA指纹图谱分析和其他形式的DNA分型。
