Department for Nutritional Toxicology, Institute of Nutrition, Friedrich-Schiller-University Jena, Dornburger Strasse 24, D-07743 Jena, Germany.
Biofactors. 2009 Sep-Oct;35(5):460-7. doi: 10.1002/biof.60.
Epidemiological studies suggest that high fish intake is associated with a decreased risk of colorectal cancer which has been linked to the high content of the n - 3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in some fish. In this study, two different cell lines are compared in relation to their response to EPA and DHA versus the plant derived PUFAs, linoleic acid (LA), gamma-linolenic acid (GLA), and alpha-linolenic acid (ALA) and to the ubiquitous arachidonic acid (ARA). The uptake of 100 microM of each fatty acid (FA) was determined using GC. The 4',6-diamidino-2-phenylindole assay for DNA quantification and the Cell-Titer-Blue assay were used to determine cell survival and metabolic activity at 2-72 h after treatment. All FAs were utilized more efficiently by the human colon adenoma cell line LT97 than by the adenocarcinoma cell line HT29. LT97 were more susceptible than HT29 cells to the growth inhibitory activities of all FAs except for DHA where both were equally sensitive. Inhibition of survival and metabolic activity by EPA and DHA increased with treatment time in both cell lines. ALA or GLA were less growth inhibitory than EPA or DHA and ARA had intermediary activity. The data show that the tested FAs are incorporated into colon cells. Furthermore, adenoma cells are more susceptible than the adenocarcinoma cells.
流行病学研究表明，高鱼类摄入量与结直肠癌风险降低有关，这与某些鱼类中 n-3 多不饱和脂肪酸（PUFAs）二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的高含量有关。在这项研究中，比较了两种不同的细胞系，以研究它们对 EPA 和 DHA 的反应与植物衍生的 PUFAs，亚油酸（LA）、γ-亚麻酸（GLA）和α-亚麻酸（ALA）以及无处不在的花生四烯酸（ARA）的反应。使用 GC 测定了每种脂肪酸（FA）100µM 的摄取量。使用 4'，6-二脒基-2-苯基吲哚（DAPI）测定法进行 DNA 定量，以及细胞 - 噻唑蓝（Cell-Titer-Blue）测定法，在处理后 2-72 小时内测定细胞存活和代谢活性。所有 FAs 都被人结肠腺瘤细胞系 LT97 比腺癌细胞系 HT29 更有效地利用。与 HT29 细胞相比，LT97 细胞对所有 FAs 的生长抑制活性更敏感，除了 DHA，两者同样敏感。在两种细胞系中，EPA 和 DHA 的存活和代谢活性抑制作用随着处理时间的增加而增加。ALA 或 GLA 的生长抑制作用低于 EPA 或 DHA，ARA 具有中间活性。数据表明，测试的 FAs 被整合到结肠细胞中。此外，腺瘤细胞比腺癌细胞更敏感。
