Agarwal Padam Kumari
Department of Pathology, King George's Medical College, Lucknow, Uttar Pradesh, India.
Acta Cytol. 2009 Sep-Oct;53(5):527-32. doi: 10.1159/000325380.
To develop a simplified technique for processing hemorrhagic body fluids, allowing elimination of red blood cells (RBCs) to obtain tumor cell-rich cytosmears for accurate diagnosis of malignancy.
Hemorrhagic fluid is collected with ethylenediamine tetraacetic acid anticoagulant. The cells are separated by centrifugation in glass capillaries into 3 layers, with the uppermost containing supernatant, middle buffy coat and lowermost dark layer of RBCs. The smears of the buffy coat are prepared by breaking the capillaries at the junction of buffy coat and RBC layer. The procedure is named capillary centrifugation technique. This procedure was developed in the cytology laboratory at King George's Medical College, Lucknow, India in 1974.
Cell yield is good and cellular details are well preserved. No cell debris is present on the slides. The background of the smears is absolutely clear. Any number of slides may be prepared for special study for exact typing of tumor cells
This is a simple, economical procedure and a good substitute for a cytocentrifuge machine for preparing smears from small amount of fluids. Technicians learn it quickly and are quite comfortable with the procedure.
开发一种用于处理出血性体液的简化技术，能够去除红细胞（RBC）以获得富含肿瘤细胞的细胞涂片，用于准确诊断恶性肿瘤。
用乙二胺四乙酸抗凝剂收集出血性液体。通过在玻璃毛细管中离心将细胞分离成三层，最上层为上清液，中间为灰白色层，最下层为红细胞的深色层。在灰白色层和红细胞层的交界处打破毛细管，制备灰白色层的涂片。该程序称为毛细管离心技术。此程序于1974年在印度勒克瑙乔治国王医学院的细胞学实验室开发。
细胞产量良好，细胞细节保存完好。载玻片上没有细胞碎片。涂片背景绝对清晰。可为肿瘤细胞的精确分型准备任意数量的载玻片用于特殊研究。
这是一种简单、经济的程序，是使用细胞离心涂片机制备少量液体涂片的良好替代方法。技术人员能很快学会，并且对该程序操作自如。
