Inbasekaran Poovizhi, Subramanian Ramaswamy Anikode
Department of Pathology, Vinayaka Mission Kirupananda Variyar Medical College and Hospital, Salem, Tamil Nadu, India.
Department of Pathology, PES Institute of Medical Sciences and Research, Chittoor, Andhra Pradesh, India.
Cytojournal. 2022 Jul 11;19:44. doi: 10.25259/Cytojournal_14_2021. eCollection 2022.
Fluids are one the most common specimens received in cytology laboratories. The presence of erythrocytes may obscure the cells in the smears, making the diagnosis, and identification of cells difficult. Many techniques are being used by laboratories to eliminate these erythrocytes. The present study was undertaken to improve the quality of cytology smears of hemorrhagic samples by comparing three different techniques, namely, Carnoy's fixative (CF), modified CF, and normal saline rehydration technique (NSRT) to hemolysis red blood cells (RBC) present in the smear background for better cytological assessment. The present study was a prospective study done over 1 year 6 months from November 2012 to March 2014, in the Department of Pathology in a Tertiary Care Rural Medical College.
All hemorrhagic effusions received in the department of pathology were processed using CF, modified CF, and NSRT. The background of the smear and cytomorphological details with two different stains was analyzed. The Chi-square test was used to find out the association of different techniques in the reduction of RBC.
More than 60% reduction of RBCs in the smear was noted in 85.40%, 14.60%, and 15.60% by NSRT, modified CF, and CF, respectively. Staining was better and nuclear features were best preserved in NSRT.
NSRT is the best, simple, and cheaper technique to lyse RBC in the hemorrhagic fluid. It also shows better staining and well-preserved cytomorphological features of the cell.
体液是细胞学实验室接收的最常见标本之一。红细胞的存在可能会使涂片上的细胞模糊不清，从而难以进行细胞诊断和识别。实验室正在使用多种技术来去除这些红细胞。本研究旨在通过比较三种不同技术，即卡诺伊固定液（CF）、改良CF和生理盐水复水技术（NSRT），来溶解涂片背景中存在的红细胞，以提高出血性样本的细胞学涂片质量，从而进行更好的细胞学评估。本研究是一项前瞻性研究，于2012年11月至2014年3月在一所三级农村医学院的病理科进行，为期1年6个月。
病理科接收的所有出血性积液均采用CF、改良CF和NSRT进行处理。分析了涂片背景以及两种不同染色方法下的细胞形态学细节。采用卡方检验来确定不同技术在减少红细胞方面的相关性。
NSRT、改良CF和CF分别使涂片中红细胞减少60%以上的比例为85.40%、14.60%和15.60%。NSRT染色效果更好，细胞核特征保存最佳。
NSRT是溶解出血性液体中红细胞的最佳、简单且成本较低的技术。它还能显示出更好的染色效果以及细胞良好保存的细胞形态学特征。
