Cao Xiaohong, Mao Dezhi, Wang Chunling, Zeng Bin, Wang Aihua, Lu Meifang, Xu Chengjian
Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
Zoolog Sci. 2009 Apr;26(4):249-53. doi: 10.2108/zsj.26.249.
A lectin with potent mitogenic activity was isolated from Musca domestica pupae. The lectin was a monomer with a molecular weight of 55 kDa. The purification procedure mainly involved affinity chromatography on galactose-Sepharose-4B. The lectin agglutinated trypsin-treated rabbit blood cells. D-galactose was detected to inhibit the lectin's hemagglutination activity. The lectin was metal independent and temperature dependent and was detected by the periodic acid-Shiff reaction to be a glycoprotein with a carbohydrate content of 2.1%. Observations with an atomic force microscope indicated that the lectin was a globular protein about 75 nm in size, with several carbohydrate chains linked to it. It elicited a mitogenic response from mouse splenocytes in vitro, with the maximal response at a concentration of 20 microg/ml. No lectin has been reported before from Musca domestica pupae, and the lectin we discovered could be of great importance in insect immunity and hypoimmune diseases.
从家蝇蛹中分离出一种具有强促有丝分裂活性的凝集素。该凝集素为单体，分子量为55 kDa。纯化过程主要包括在半乳糖 - 琼脂糖 - 4B上进行亲和层析。该凝集素能凝集经胰蛋白酶处理的兔血细胞。检测到D - 半乳糖可抑制该凝集素的血凝活性。该凝集素不依赖金属而依赖温度，经高碘酸 - 希夫反应检测为糖蛋白，碳水化合物含量为2.1%。原子力显微镜观察表明，该凝集素是一种大小约为75 nm的球状蛋白，有几条碳水化合物链与之相连。它在体外能引起小鼠脾细胞的促有丝分裂反应，在浓度为20 μg/ml时反应最大。此前尚未有家蝇蛹凝集素的报道，我们发现的这种凝集素在昆虫免疫和免疫低下疾病中可能具有重要意义。
