Sun Xue-Jun, Sun Feng-Lei, Du Jun-Kai, Pan Qing-Hua, Zhang Yuan-Fu, Xu Yi-Zhuang, Shi Jing-Sen, Wu Jin-Guang
The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Jul;29(7):1750-3.
The aim of the present research is to establish the cell basis for the carcinoma tissue diagnosis by exploring a method to obtain the FTIR (Fourier transform infrared spectra) of the cultured carcinoma cell and nucleus with FTIR spectroscopy, and investigating the special spectral features of the carcinoma cell and nucleus compared with the carcinoma tissues. In this paper, the gallbladder carcinoma tissues confirmed by histology were measured using a Nicolet Magna 5700-II FTIR spectrometer and the corresponding FTIR spectra were obtained. The cultured gallbladder carcinoma cell (GBC-SD) and nucleus were centrifuged to provide a small pellet of cell and nucleus for FTIR analysis. The cell and nucleus pellet was then placed on the OMNIC sampler. Then the infrared spectra were recorded by the same equipment. Based on the previously established criteria, a comparative study was subsequently carried out between the spectra of the cultured carcinoma cell and nucleus (GBC-SD) and that of the corresponding gallbladder tissues. Several infrared spectral features of the carcinoma cell and nucleus were obtained. All the results suggest that the spectral features of the carcinoma cell and nucleus can be well reflected by that of the carcinoma tissue, though the later is more complicated, which might originate from the intrinsic complexity of the tissue. This study shows that the diagnosis of carcinoma tissue by FTIR method exhibits sufficient cell basis.
本研究的目的是通过探索一种利用傅里叶变换红外光谱法获取培养的癌细胞和细胞核的傅里叶变换红外光谱（FTIR）的方法，并研究癌细胞和细胞核与癌组织相比的特殊光谱特征，来建立癌组织诊断的细胞基础。在本文中，使用Nicolet Magna 5700-II傅里叶变换红外光谱仪对经组织学证实的胆囊癌组织进行测量，并获得相应的傅里叶变换红外光谱。将培养的胆囊癌细胞（GBC-SD）和细胞核离心，以提供一小团细胞和细胞核用于傅里叶变换红外分析。然后将细胞和细胞核沉淀放置在OMNIC采样器上。接着用同一设备记录红外光谱。基于先前确立的标准，随后对培养的癌细胞和细胞核（GBC-SD）的光谱与相应胆囊组织的光谱进行了对比研究。获得了癌细胞和细胞核的几个红外光谱特征。所有结果表明，尽管癌组织的光谱特征更复杂，可能源于组织固有的复杂性，但癌细胞和细胞核的光谱特征能够被癌组织的光谱特征很好地反映出来。本研究表明，通过傅里叶变换红外光谱法诊断癌组织具有充分的细胞基础。
