Du Jun-Kai, Shi Jing-Sen, Sun Xue-Jun, Wang Jian-Sheng, Xu Yi-Zhuang, Wu Jin-Guang, Zhang Yuan-Fu, Weng Shi-Fu
Department of General Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
Hepatobiliary Pancreat Dis Int. 2009 Feb;8(1):75-8.
Fourier transform infrared (FT-IR) spectroscopy is a physical method applied to the study of cellular changes at the molecular level in various normal and diseased human tissues, including cancer. This study was undertaken to establish a cellular basis for the diagnosis of carcinoma tissue, using FT-IR spectroscopy to study a carcinoma cell line and investigating the specific spectral features of the cell line.
The FT-IR spectra of cultured gallbladder carcinoma cells (GBC-SD) smeared on a BaF2 window were measured with a Nicolet Magna750-II FT-IR spectrometer. A comparative study was subsequently carried out between the spectra of cultured gallbladder carcinoma cells and those of corresponding carcinoma tissue.
Several infrared spectral features were obtained, and the results suggest that the spectral features of the carcinoma cell line reflect those of carcinoma tissue, though the latter are more complex, probably due to the intrinsic complexity of the tissue.
The diagnosis of carcinoma tissue by FT-IR spectroscopy has a sufficient cellular basis.
傅里叶变换红外（FT-IR）光谱学是一种物理方法，应用于研究包括癌症在内的各种正常和患病人体组织在分子水平上的细胞变化。本研究旨在通过使用FT-IR光谱学研究癌细胞系并探究该细胞系的特定光谱特征，为癌组织的诊断建立细胞基础。
使用Nicolet Magna750-II傅里叶变换红外光谱仪测量涂抹在BaF2窗口上的培养胆囊癌细胞（GBC-SD）的FT-IR光谱。随后对培养的胆囊癌细胞光谱与相应癌组织光谱进行了对比研究。
获得了几个红外光谱特征，结果表明癌细胞系的光谱特征反映了癌组织的光谱特征，尽管后者更为复杂，这可能是由于组织本身的复杂性所致。
通过傅里叶变换红外光谱学诊断癌组织具有充分的细胞基础。
