Sun Gong-Lei, Yu Peng, Ruan Qin, Li Dan-Ting, Cheng Cun-Gui
Zhejiang Key Laboratory for Reactive Chemistry on Solid Surfaces, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Jul;29(7):1822-5.
In the present paper, FTIR of different species obtained from different regions of sibling plantaginis were determined by Fourier transform infrared reflection spectroscopy (FTIRS), and thirty-five comparatively typical absorption peaks were selected and used to study genetic relationship, combined with chemometric methods. The phylogenetic cluster analysis revealed that three species could be divided into two groups based on the distance of 0.036, among which Plantago depressa Willd. was clustered with Plantago asiatica L. based on distance of 0.033, while clustered with Plantago virginica L. based on distance of 0.042, and the result was consistent with that of traditional taxology. The principal component analysis result revealed that the distances of Plantago asiatica L. in the similar environment are similar in three-dimensional FTIR chart, however, it is dispersive when obtained from different regions. This method is scientific, simple and direct, and has important theoretical and practical application value.
本文采用傅里叶变换红外反射光谱法（FTIRS）测定了同属车前草不同地区的不同种的傅里叶变换红外光谱（FTIR），选取35个较为典型的吸收峰并结合化学计量学方法研究亲缘关系。系统聚类分析结果表明，在距离为0.036时，三种植物可分为两组，其中平车前与车前在距离为0.033时聚类在一起，而与北美车前在距离为0.042时聚类在一起，结果与传统分类学一致。主成分分析结果表明，车前在相似环境下的三维FTIR图谱中距离相近，但从不同地区获取时则较为分散。该方法科学、简便、直接，具有重要的理论和实际应用价值。
