车前子多糖的化学特性及抗氧化活性研究
Chemical characteristics and antioxidant activities of polysaccharide purified from the seeds of Plantago asiatica L.
机构信息
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
出版信息
J Sci Food Agric. 2010 Jan 30;90(2):210-7. doi: 10.1002/jsfa.3793.
BACKGROUND
A water-soluble polysaccharide from the seeds of Plantago asiatica L. (P. asiatica L. polysaccharide, PLP) was extracted with hot water and purified by gel filtration chromatography. The chemical characteristics of PLP were determined by high-performance gel permeation chromatography (HPGPC) and Fourier transform infrared (FTIR) spectroscopy. In addition, the antioxidant activities of PLP in vitro were evaluated using various test systems, including scavenging of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radicals, scavenging of superoxide radicals generated by 1,2,3-phentriol autoxidation, scavenging of hydroxyl radicals and inhibition of lipid peroxidation.
RESULTS
The molecular weight of PLP was determined by HPGPC to be about 1894 kDa. PLP contained 29.2 g kg(-1) protein and 145.8 g kg(-1) uronic acid. The FTIR spectrum of PLP also revealed typical characteristics of a polysaccharide containing protein and uronic acid. Moreover, the results showed that PLP possessed antioxidant activities, but lower than those of ascorbic acid.
CONCLUSION
PLP is an acid protein-bound polysaccharide of high molecular weight, but its structure needs further study. The present results suggest that PLP could potentially be used as a natural antioxidant.
背景
车前子种子中的一种水溶性多糖（车前子多糖，PLP），经热水提取并通过凝胶过滤色谱法纯化得到。通过高效凝胶渗透色谱（HPGPC）和傅里叶变换红外（FTIR）光谱确定了 PLP 的化学特性。此外，还通过各种测试系统评估了 PLP 的体外抗氧化活性，包括清除 1,1-二苯基-2-苦基肼基（DPPH）自由基、清除 1,2,3-苯三醇自氧化产生的超氧自由基、清除羟自由基和抑制脂质过氧化。
结果
通过 HPGPC 确定 PLP 的分子量约为 1894 kDa。PLP 含有 29.2 g kg(-1)的蛋白质和 145.8 g kg(-1)的糖醛酸。PLP 的 FTIR 光谱也显示出含有蛋白质和糖醛酸的多糖的典型特征。此外，结果表明 PLP 具有抗氧化活性，但低于抗坏血酸。
结论
PLP 是一种高分子量的酸性蛋白结合多糖，但需要进一步研究其结构。本研究结果表明，PLP 可能具有作为天然抗氧化剂的潜力。
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