Guo Jun-tao, Zhou Yi-biao, Zhang Zhi-jie, Liu Gang-ming, Yihuo Wu-li, Wang Hai-yin, Zhao Gen-ming
Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2009 May;30(5):497-501.
To analysis the spatial autocorrelation on the small-scale distribution of the genetic variation in the population of Oncomelania hupensis in Puge county, Sichuan province, using simple sequence repeat (SSR) marker.
5 pairs of SSR primer were used to amplify the genomic DNA of Oncomelania hupensis, and the alleles with frequency ranging from 15% to 85% were used to calculate Moran's I spatial autocorrelation coefficients in 14 distance band based on equal numbers of paired samples.
A total of 274 alleles were scored by 5 pairs of SSR primer, the average polymorphic information content of the 274 alleles were 0.965 which indicated a high level of genetic diversity. 39 alleles showed different patterns of positive spatial autocorrelation of genetic variation, which was non-random spatial structure. When the distance band increased, the spatial auto-correlativity decreased based on the average Moran's I value at 14 distance band. The alleles which showed a negative spatial autocorrelation were not found in any distance band.
The spatial distribution of the genetic variation of SSR showed positive spatial autocorrelation in the population of Oncomelania hupensis, and the spatial auto-correlativity decreased with the increase of distance band.
利用简单序列重复（SSR）标记分析四川省普格县钉螺种群遗传变异小尺度分布的空间自相关性。
采用5对SSR引物扩增钉螺基因组DNA，基于等数量配对样本，对频率范围在15%至85%的等位基因在14个距离带中计算莫兰指数（Moran's I）空间自相关系数。
5对SSR引物共检测到274个等位基因，274个等位基因的平均多态信息含量为0.965，表明遗传多样性水平较高。39个等位基因呈现出不同模式的遗传变异正空间自相关性，即非随机空间结构。基于14个距离带的平均莫兰指数值，当距离带增加时，空间自相关性降低。在任何距离带均未发现呈现负空间自相关性的等位基因。
SSR遗传变异的空间分布在钉螺种群中呈现正空间自相关性，且空间自相关性随距离带增加而降低。
