Li Shi-Zhu, Zhang L, Liu Qin, Lv Shan, Wang Qiang, Qian Ying-Jun, Yang Kun, Zhou Xiao-Nong
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, MOH, WHO Collaborating Center of Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2012 Aug 30;30(4):268-73.
To identify the genetic structure of Oncomelania hupensis in the middle and lower reaches of Yangtze River by using microsatellite DNA molecular markers.
O. hupensis snails were collected from the provinces of Anhui, Hunan, Hubei, Jiangxi, and Jiangsu, of which 6 polymorphic microsatellite DNA loci (P84, T5-13, T5-11, T4-22, T6-27 and P82) were amplified with fluorescence labeled universal primer. 20-50 snail samples were collected at each spot, adding up to 165 samples. The number of alleles (N(alpha)), inbreeding coefficient (F(IS)), heterozygosity (H), fixation index (F(ST)) of each group snails, genetic distance between/groups, and the polymorphic information content (PIC) were calculated. Cluster analysis was then carried out based on genetic distance, and hierarchical AMOVA calculation was done.
The number of alleles in each population ranged from 3 to 33, and the inbreeding coefficient ranged from 0.143 to 0.539. The average expected heterozygosity and observed heterozygosity ranged from 0.600 to 0.883 and 0.308 to 0.759, respectively, being the highest in Hubei population and the lowest in Jiangsu population. The range of F(ST) value between paired populations was from 0.0006 to 0.0531. The small F(ST) value suggested that genetic differentiation did not occur among the populations. The average polymorphic information content in the populations ranged from 0.511 to 0.850, showing a high polymorphism except the Jiangsu population. Hierarchical AMOVA calculations showed that inter-individual variation of the snails occupied 95.2% of the total variations. Cluster analysis revealed that Anhui group clustered first to Jiangsu, followed with Hunan, Jiangxi and Hubei.
There is a rich diversity in O. hupensis. Cluster analysis shows a consistency with the geographical distribution pattern. The genetic differences among the 5 snail populations are trivial with microsatellite DNA variation mostly present in individuals.
利用微卫星DNA分子标记鉴定长江中下游地区湖北钉螺的遗传结构。
从安徽、湖南、湖北、江西和江苏等省采集湖北钉螺，采用荧光标记通用引物扩增6个多态性微卫星DNA位点（P84、T5 - 13、T5 - 11、T4 - 22、T6 - 27和P82）。每个采样点采集20 - 50只钉螺样本，共165个样本。计算每组钉螺的等位基因数（N(α)）、近交系数（F(IS)）、杂合度（H）、固定指数（F(ST)）、组间遗传距离以及多态信息含量（PIC）。然后基于遗传距离进行聚类分析，并进行层次AMOVA计算。
各群体的等位基因数在3至33之间，近交系数在0.143至0.539之间。平均期望杂合度和观察杂合度分别在0.600至0.883和0.308至0.759之间，其中湖北群体最高，江苏群体最低。配对群体间F(ST)值范围为0.0006至0.0531。较小的F(ST)值表明群体间未发生遗传分化。各群体的平均多态信息含量在0.511至0.850之间，除江苏群体外均表现出高度多态性。层次AMOVA计算表明，钉螺个体间变异占总变异的95.2%。聚类分析显示，安徽群体首先与江苏群体聚类，其次是湖南、江西和湖北群体。
湖北钉螺具有丰富的多样性。聚类分析结果与地理分布格局一致。5个钉螺群体间的遗传差异较小，微卫星DNA变异主要存在于个体中。
