Matsuno Y, Hirohashi S, Furuya S, Sakamoto M, Mukai K, Shimosato Y
Pathology Division, National Cancer Center Research Institute, Tokyo.
Jpn J Cancer Res. 1990 Nov;81(11):1137-40. doi: 10.1111/j.1349-7006.1990.tb02525.x.
Surgically resected small hepatocellular carcinomas showing "nodule-in-nodule" formation were analyzed in terms of cell proliferative activity. The analysis was achieved by successful immunohistochemical demonstration of proliferating cell nuclear antigen in formalin-fixed paraffin-embedded tissue sections. Eight nodules (up to 3 cm in diameter) examined were either atypical adenomatous hyperplasia or hepatocellular carcinoma of low histologic grade, containing a discrete inner nodular area composed of obvious hepatocellular carcinoma of higher histologic grade. In all cases, the proliferating cell nuclear antigen labeling index of the latter area was much higher than that of the former, which in turn was slightly higher than that of the non-cancerous liver of the patient in 6 cases. The data presented here provide supporting evidence that the successive emergence and expansion of a more rapidly proliferating subclone within a nodule result in the stepwise progression of malignancy of human hepatocellular carcinoma.
对手术切除的呈现“结节内结节”形成的小肝细胞癌进行细胞增殖活性分析。通过在福尔马林固定石蜡包埋组织切片中成功进行增殖细胞核抗原的免疫组织化学显示来实现该分析。所检查的8个结节（直径达3厘米）要么是非典型腺瘤样增生，要么是低组织学分级的肝细胞癌，包含一个由明显的高组织学分级肝细胞癌组成的离散内部结节区域。在所有病例中，后一区域的增殖细胞核抗原标记指数远高于前一区域，而前一区域又有6例略高于患者的非癌肝脏。此处呈现的数据提供了支持性证据，表明结节内增殖更快的亚克隆的连续出现和扩展导致人类肝细胞癌恶性程度的逐步进展。
