Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calz México-Xochimilco 101, Col San Lorenzo Huipulco, 14370 México, DF, Mexico.
J Ethnopharmacol. 2010 Jan 8;127(1):91-7. doi: 10.1016/j.jep.2009.09.044. Epub 2009 Sep 30.
Around the world, Tilia species have been used in traditional medicine for their properties as tranquilizer. Furthermore, Mexican species of Tilia have been grouped as Tilia americana var. mexicana, but their specific content in flavonoids is poorly described. In this study, inflorescences of Mexican Tilia were collected in three different regions of Mexico to compare their flavonoid content and anxiolytic-like response.
Flavonoid content was analyzed by using an HPLC-MS technique. For anxiolytic-like response, Tilia inflorescences extracts (from 10 to 300 mg/kg, i.p.) were tested in experimental models (open-field, hole-board and plus-maze tests, as well as sodium pentobarbital-induced hypnosis) in mice.
HPLC-MS analysis revealed specific peaks of flavonoid composition demonstrating some differences in these compounds in flowers and bracts depending on the region of collection. No differences in the neuropharmacological activity among these samples of Tilia were found. Moreover, their effects were associated with quercetin and kaempferol glycosides.
Dissimilarities in the flavonoid composition of Mexican Tilias might imply that these species must be re-classified in more than one species, not as a unique Tilia americana var. mexicana. Since quercetin and kaempferol aglycons demonstrated anxiolytic-like response and that no difference in the pharmacological evaluation was observed between these three Mexican Tilias, we suggest that this pharmacological effect of Tilia inflorescences involves these flavonoids occurrence independently of the kind of glycosides present in the samples reinforcing their use in traditional medicine in several regions of Mexico.
在世界各地，丁香属植物因其具有镇静作用而被用于传统医学。此外，墨西哥的丁香属植物被归类为美国丁香的墨西哥变种，但它们的类黄酮含量描述得很差。在这项研究中，收集了来自墨西哥三个不同地区的墨西哥丁香花序，以比较它们的类黄酮含量和抗焦虑样反应。
使用 HPLC-MS 技术分析类黄酮含量。对于抗焦虑样反应，将丁香花序提取物（10 至 300mg/kg，ip）在实验模型（旷场、洞板和十字迷宫试验以及戊巴比妥钠诱导催眠）中测试在小鼠中。
HPLC-MS 分析显示出类黄酮组成的特定峰，表明这些化合物在花朵和苞片中因采集地点的不同而存在一些差异。在这些丁香样品中，神经药理学活性没有差异。此外，它们的作用与槲皮素和山柰酚糖苷有关。
墨西哥丁香的类黄酮组成的差异可能意味着这些物种必须重新分类为不止一个物种，而不是作为一个独特的美国丁香的墨西哥变种。由于槲皮素和山柰酚苷元表现出抗焦虑样反应，并且在这三种墨西哥丁香之间没有观察到药理评价的差异，我们建议丁香花序的这种药理作用涉及到这些类黄酮的存在，而与样品中存在的糖苷类型无关，这加强了它们在墨西哥几个地区传统医学中的应用。
