Coballase-Urrutia Elvia, Cárdenas-Rodríguez Noemí, Carmona-Aparicio Liliana, Sánchez-Valle Vicente, Rivera-Espinosa Liliana, Pedraza-Chaverri José, Montesinos-Correa Hortencia, Bello-Robles Edith, Sampieri Aristides Iii, Martínez-Vargas David, Granados-Rojas Leticia, González-Trujano María Eva
Laboratory of Neuroscience, National Institute of Pediatrics, Mexico City, Mexico.
Neuroplasticity and Neurodegeneration Laboratory, Department of Pharmacology, Center for Research and Advanced Studies, Mexico City, Mexico.
Iran J Pharm Res. 2022 May 3;21(1):e126914. doi: 10.5812/ijpr-126914. eCollection 2022 Dec.
var. possesses anticonvulsant, antioxidant, neuroprotective, and hepatoprotective activities. The spectrum of anticonvulsant activity in status epilepticus models has not been sufficiently explored. We evaluated the effects of ethyl acetate (EAc), and methanol (ME) extracts on kainic acid (KA)-induced seizures by measuring rats'behavior (severity and latency) and lipoperoxidation in different brain areas (cerebellum, brain hemispheres, cortex, and medulla), kidneys, and liver. Male Wistar rats were administered KA (10 mg/kg, i.p.) after three days of pretreatment with extract (100 mg/kg). The EAc and ME extracts significantly decreased the severity of phase 1 and phase 2 seizures, respectively. The ME extract increased the latency to seizure (27 ± 2 min) compared to the control (13 ± 2 min). The ME and EAc extracts significantly prevented the increased lipid peroxidation caused by KA-induced seizures in the cerebellum, brain hemispheres, cortex, medulla, liver, and kidneys. The vehicle olive oil (OO) also showed anticonvulsant effects, decreasing the severity of seizures to phase 3 and lipoperoxidation levels in the cerebellum, brain hemispheres, cortex, medulla, liver, and kidneys. The anticonvulsant activity of is mediated by antioxidant effects in central and systemic areas that involve synergistic interactions among the chemical constituents of these extracts (glucosides of quercetin and kaempferol), while vehicle OO showed the same effects, probably due to its constituent oleuropein.
变种具有抗惊厥、抗氧化、神经保护和肝脏保护活性。癫痫持续状态模型中的抗惊厥活性谱尚未得到充分探索。我们通过测量大鼠的行为（严重程度和潜伏期）以及不同脑区（小脑、脑半球、皮层和延髓）、肾脏和肝脏中的脂质过氧化，评估了乙酸乙酯（EAc）和甲醇（ME）提取物对 kainic 酸（KA）诱导的癫痫发作的影响。雄性 Wistar 大鼠在预处理三天后（提取物 100mg/kg）腹腔注射 KA（10mg/kg）。EAc 和 ME 提取物分别显著降低了 1 期和 2 期癫痫发作的严重程度。与对照组（13±2 分钟）相比，ME 提取物延长了癫痫发作的潜伏期（27±2 分钟）。ME 和 EAc 提取物显著预防了 KA 诱导的癫痫发作在小脑、脑半球、皮层、延髓、肝脏和肾脏中引起的脂质过氧化增加。载体橄榄油（OO）也显示出抗惊厥作用，将癫痫发作的严重程度降低到 3 期，并降低了小脑、脑半球、皮层、延髓、肝脏和肾脏中的脂质过氧化水平。的抗惊厥活性是由中枢和全身区域的抗氧化作用介导的，这些区域涉及这些提取物（槲皮素和山奈酚的糖苷）化学成分之间的协同相互作用，而载体 OO 显示出相同的效果，可能是由于其成分橄榄苦苷。
